Zhi-Bin Liu1, Wen-min Niu. 1. Shaanxi College of Chinese Medicine, Xianyang 712083, China. lzb210396@163.com
Abstract
OBJECTIVE: To explore the protective effect of electroacupuncture (EA) of "Xiusanzhen"([Chinese characters: see text]) on mitochondria of cortical neurons in acute cerebral ischemia-reperfusion (CI/R) rats. METHODS: A total of 30 cases of SD rats were divided into control, model and EA groups. CI/R model was established by occlusion of the middle cerebral artery and reperfusion. EA (80-100 Hz, 1-3 mA) was applied to "Xiusanzhen" (the median line of the nose root, 2 mm to the lateral median line on the bilateral sides) for 60 min. Content of serum reduced glutathione (GSH) and glutathion peroxidase (GSH-Px) activity were detected by using Tietze's reducase assay and dithio-bis-nitrobenzoic acid (DTNB) direct method respectively. The surface density (Sv), numerical density (Nv) and perimeter/area (Pv) of the mitochondria of the cerebral neurons were detected using stereological technique and electronic microscope. RESULTS: Compared with control group, Sv and Pv of model group increased significantly (P < 0.05), while serum GSH content and GSH-Px activity of model group decreased considerably (P < 0.05). While in comparison with model group, Sv and Pv of EA group lowered obviously (P < 0.05), and GSH and GSH-Px both increased pronouncedly (P < 0.05). No significant difference was found among 3 groups in Nv (P > 0.05). CONCLUSION: EA of "Xiusanzhen" has a definite protective effect on mitochondria of cortical neurons in acute focal cerebral ischemia-reperfusion rats, which may be related with its antioxidation.
OBJECTIVE: To explore the protective effect of electroacupuncture (EA) of "Xiusanzhen"([Chinese characters: see text]) on mitochondria of cortical neurons in acute cerebral ischemia-reperfusion (CI/R) rats. METHODS: A total of 30 cases of SD rats were divided into control, model and EA groups. CI/R model was established by occlusion of the middle cerebral artery and reperfusion. EA (80-100 Hz, 1-3 mA) was applied to "Xiusanzhen" (the median line of the nose root, 2 mm to the lateral median line on the bilateral sides) for 60 min. Content of serum reduced glutathione (GSH) and glutathion peroxidase (GSH-Px) activity were detected by using Tietze's reducase assay and dithio-bis-nitrobenzoic acid (DTNB) direct method respectively. The surface density (Sv), numerical density (Nv) and perimeter/area (Pv) of the mitochondria of the cerebral neurons were detected using stereological technique and electronic microscope. RESULTS: Compared with control group, Sv and Pv of model group increased significantly (P < 0.05), while serum GSH content and GSH-Px activity of model group decreased considerably (P < 0.05). While in comparison with model group, Sv and Pv of EA group lowered obviously (P < 0.05), and GSH and GSH-Px both increased pronouncedly (P < 0.05). No significant difference was found among 3 groups in Nv (P > 0.05). CONCLUSION: EA of "Xiusanzhen" has a definite protective effect on mitochondria of cortical neurons in acute focal cerebral ischemia-reperfusion rats, which may be related with its antioxidation.