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Literature DB >> 17691104

Fluorophore-labeled myosin-specific antibodies simplify muscle-fiber phenotyping.

Paul Gregorevic¹, Norman A Meznarich, Michael J Blankinship, Robert W Crawford, Jeffrey S Chamberlain.

Abstract

Skeletal muscles are frequently analyzed for composition of phenotypically distinct myofibers, as a functional determinant. We describe an improved myofiber phenotyping procedure, involving cryosection co-incubation with fluorophore-labeled myosin heavy-chain (MyHC)-isoform-specific antibodies. This technique identifies multiple fiber "types" on a single section, thereby reducing reagents and processing, and offers side-by-side comparison of samples from multiple species including mice. These advances are valuable for studying the physiological attributes of skeletal muscle in health and disease.

Entities: Gene Species

Mesh：

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Year: 2008 PMID： 17691104 PMCID： PMC2788960 DOI： 10.1002/mus.20877

Source DB: PubMed Journal: Muscle Nerve ISSN： 0148-639X Impact factor: 3.217

6 in total

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5. Expression of myosin heavy chain isoforms in Duchenne muscular dystrophy patients and carriers.

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6. A new blocking method for application of murine monoclonal antibody to mouse tissue sections.

Authors: Q L Lu; T A Partridge
Journal: J Histochem Cytochem Date: 1998-08 Impact factor: 2.479

6 in total

16 in total

1. SMASH - semi-automatic muscle analysis using segmentation of histology: a MATLAB application.

Authors: Lucas R Smith; Elisabeth R Barton
Journal: Skelet Muscle Date: 2014-11-27 Impact factor: 4.912

2. Yap regulates skeletal muscle fatty acid oxidation and adiposity in metabolic disease.

Authors: K I Watt; D C Henstridge; M Ziemann; C B Sim; M K Montgomery; D Samocha-Bonet; B L Parker; G T Dodd; S T Bond; T M Salmi; R S Lee; R E Thomson; A Hagg; J R Davey; H Qian; R Koopman; A El-Osta; J R Greenfield; M J Watt; M A Febbraio; B G Drew; A G Cox; E R Porrello; K F Harvey; P Gregorevic
Journal: Nat Commun Date: 2021-05-17 Impact factor: 14.919

3. Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse.

Authors: Tomohiro Suga; En Kimura; Yuka Morioka; Masahito Ikawa; Sheng Li; Katsuhisa Uchino; Yuji Uchida; Satoshi Yamashita; Yasushi Maeda; Jeffrey S Chamberlain; Makoto Uchino
Journal: PLoS One Date: 2011-03-18 Impact factor: 3.240

4. Differentiation and fiber type-specific activity of a muscle creatine kinase intronic enhancer.

Authors: Phillip Wl Tai; Katherine I Fisher-Aylor; Charis L Himeda; Catherine L Smith; Alexandra P Mackenzie; Deri L Helterline; John C Angello; Robert E Welikson; Barbara J Wold; Stephen D Hauschka
Journal: Skelet Muscle Date: 2011-07-07 Impact factor: 4.912

Fluorophore-labeled myosin-specific antibodies simplify muscle-fiber phenotyping.

1. Force-velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle.

2. Monospecific antibodies against the three mammalian fast limb myosin heavy chains.

3. Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.

4. Embryonic and neonatal myosin heavy chain in denervated and paralyzed rat skeletal muscle.

5. Expression of myosin heavy chain isoforms in Duchenne muscular dystrophy patients and carriers.

6. A new blocking method for application of murine monoclonal antibody to mouse tissue sections.

1. SMASH - semi-automatic muscle analysis using segmentation of histology: a MATLAB application.

2. Yap regulates skeletal muscle fatty acid oxidation and adiposity in metabolic disease.

3. Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse.

4. Differentiation and fiber type-specific activity of a muscle creatine kinase intronic enhancer.

Review 5. History and development of staining methods for skeletal muscle fiber types.

6. Systemic RNAi delivery to the muscles of ROSA26 mice reduces lacZ expression.

7. Muscle structure influences utrophin expression in mdx mice.

8. FHL1 activates myostatin signalling in skeletal muscle and promotes atrophy.

9. Differential myofiber-type transduction preference of adeno-associated virus serotypes 6 and 9.

10. Patterns of Age-Associated Degeneration Differ in Shoulder Muscles.