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Literature DB >> 17682835

Germ line transmission and expression of an RNAi cassette in mice generated by a lentiviral vector system.

Milen Kirilov¹, Minqiang Chai, Frank van der Hoeven, Ulrich Kloz, Wolfgang Schmid, Günther Schütz.

Abstract

We have used a lentiviral delivery system (LentiLox3.7) to generate transgenic mice harbouring RNA interference (RNAi) against the hepatocyte nuclear factor 4 gamma (HNF4gamma). HNF4gamma is a nuclear receptor with unknown function. Our analyses performed on founder (F(0)) and first generation (F(1)) mice revealed mosaicism in F(0) founders and a low efficiency of transgenesis (6%) in F(1) mice. These data, together with the observation of multiple silenced transgenes, do not favour the use of LentiLox3.7 lentivirus for transgenesis. Despite the low efficiency of transgenesis, we achieved a tissue-dependent knockdown of HNF4gamma expression in some mice.

Entities: Chemical

Mesh：

Substances：

Year: 2007 PMID： 17682835 DOI： 10.1007/s11248-007-9119-6

Source DB: PubMed Journal: Transgenic Res ISSN： 0962-8819 Impact factor: 3.145

40 in total

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Journal: Science Date: 2002-01-10 Impact factor: 47.728

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9. Production of transgenic rats by lentiviral transduction of male germ-line stem cells.

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4 in total

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Journal: Mol Ther Date: 2012-03-20 Impact factor: 11.454