| Literature DB >> 17681764 |
Ming Qin1, Sen Hou, Likai Wang, XiZeng Feng, Rui Wang, Yanlian Yang, Chen Wang, Lei Yu, Bin Shao, MingQiang Qiao.
Abstract
Protein immobilization is a crucial step in protein chip, biosensor, etc. Here, two methods to immobilize proteins on glass surface were analyzed, one is silanization method using 3-aminopropyltriethoxysilane (APTES), and the other is hydrophobin HFBI coating. The modified glass surfaces were characterized with X-ray photoelectron spectroscopy (XPS), water contact angle measurement (WCA) and immunoassay. The results of XPS and WCA illustrated that the surface property of glass can be changed by both the two methods. The following immunoassay using microcontact printing (microCP) verified that both methods could help protein immobilization effectively on glass slides. Compared with the amine treatment, it is concluded that hydrophobin self-assemblies is a simple and generic way for protein immobilization on glass slides, which has potential application in protein chips and biosensors.Entities:
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Year: 2007 PMID： 17681764 DOI： 10.1016/j.colsurfb.2007.06.018
Source DB: PubMed Journal: Colloids Surf B Biointerfaces ISSN： 0927-7765 Impact factor: 5.268