| Literature DB >> 17681624 |
Chien-Yen Chen1, Simon C Baker, Richard C Darton.
Abstract
The presence of biosurfactants in growth media can be evaluated by a variety of methods, none of which are suitable for high throughput studies. The method described here is based on the effect of meniscus shape on the image of a grid viewed through the wells of a 96-well plate. The efficacy of the method was demonstrated by the selection of a bacterium (producing a biosurfactant able to reduce the surface tension of pure water from 72 to 28.75 mN m(-1)) from a culture collection isolated from aviation fuel-contaminated land. The assay was found to be more sensitive, rapid and easy to perform than other published methods. It does not need specialised equipment or chemicals and excludes the bias which results from the surfactant properties of medium used for bacterial growth.Entities:
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Year: 2007 PMID： 17681624 DOI： 10.1016/j.mimet.2007.06.006
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363