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Literature DB >> 1768149

Recovery of an integration shuttle vector from tandem repeats in Methanococcus maripaludis.

Abstract

Transformation of Methanococcus maripaludis by using an integration vector, pKAS102, is described. Selection and subsequent growth at high concentrations of puromycin caused pKAS102 to develop tandem repeats within the genome. As a result, total DNA isolated from the transformant could be used to recover the intact vector by direct transformation of competent Escherichia coli.

Entities: Chemical Species

Mesh：

Substances：
DNA, Bacterial

Year: 1991 PMID： 1768149 PMCID： PMC183653 DOI： 10.1128/aem.57.9.2762-2763.1991

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

5 in total

1. Genetic transformation in the methanogen Methanococcus voltae PS.

Authors: G Bertani; L Baresi
Journal: J Bacteriol Date: 1987-06 Impact factor: 3.490

2. Construction of an integration vector for use in the archaebacterium Methanococcus voltae and expression of a eubacterial resistance gene.

Authors: P Gernhardt; O Possot; M Foglino; L Sibold; A Klein
Journal: Mol Gen Genet Date: 1990-04

3. Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg.

Authors: V E Worrell; D P Nagle; D McCarthy; A Eisenbraun
Journal: J Bacteriol Date: 1988-02 Impact factor: 3.490

4. A newly-isolated marine methanogen harbors a small cryptic plasmid.

Authors: A G Wood; W B Whitman; J Konisky
Journal: Arch Microbiol Date: 1985-08 Impact factor: 2.552

5. Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger.

Authors: R A Lacalle; D Pulido; J Vara; M Zalacaín; A Jiménez
Journal: Gene Date: 1989-07-15 Impact factor: 3.688

5 in total

14 in total

1. Characterization of pURB500 from the archaeon Methanococcus maripaludis and construction of a shuttle vector.

Authors: D L Tumbula; T L Bowen; W B Whitman
Journal: J Bacteriol Date: 1997-05 Impact factor: 3.490

2. Improved and versatile transformation system allowing multiple genetic manipulations of the hyperthermophilic archaeon Thermococcus kodakaraensis.

Authors: Takaaki Sato; Toshiaki Fukui; Haruyuki Atomi; Tadayuki Imanaka
Journal: Appl Environ Microbiol Date: 2005-07 Impact factor: 4.792

7. Advances in development of a genetic system for Thermoanaerobacterium spp.: expression of genes encoding hydrolytic enzymes, development of a second shuttle vector, and integration of genes into the chromosome.

Authors: V Mai; J Wiegel
Journal: Appl Environ Microbiol Date: 2000-11 Impact factor: 4.792

8. Genetics of nitrogen regulation in Methanococcus maripaludis.

Authors: P S Kessler; J A Leigh
Journal: Genetics Date: 1999-08 Impact factor: 4.562

9. Isolation of acetate auxotrophs of the methane-producing archaeon Methanococcus maripaludis by random insertional mutagenesis.

Authors: W Kim; W B Whitman
Journal: Genetics Date: 1999-08 Impact factor: 4.562

10. Genetics in methanogens: transposon insertion mutagenesis of a Methanococcus maripaludis nifH gene.

Authors: C E Blank; P S Kessler; J A Leigh
Journal: J Bacteriol Date: 1995-10 Impact factor: 3.490