BACKGROUND AND AIMS: The transcription factor SOX9 has been shown previously to have an essential role in the differentiation of a small number of discrete cell lineages. In the intestine, Sox9 is expressed in the epithelial cells of the crypts and is a target of Wnt signaling. METHODS: To examine the function of SOX9 in the intestine, we inactivated the Sox9 gene in intestinal epithelial cells by generating mice that harbored a conditional Sox9 gene and a Villin-Cre transgene. RESULTS: In the absence of SOX9, Paneth cells were not formed, but the differentiation of other intestinal epithelial cell types was unaffected. The lack of SOX9 also lead to crypt enlargement, to a marked increase in cell proliferation throughout the crypts, and to replacement of the Paneth cells by proliferating epithelial cells. CONCLUSIONS: We conclude that SOX9 is required for the differentiation of Paneth cells. Our results elucidate an essential step in the differentiation of gut epithelium.
BACKGROUND AND AIMS: The transcription factor SOX9 has been shown previously to have an essential role in the differentiation of a small number of discrete cell lineages. In the intestine, Sox9 is expressed in the epithelial cells of the crypts and is a target of Wnt signaling. METHODS: To examine the function of SOX9 in the intestine, we inactivated the Sox9 gene in intestinal epithelial cells by generating mice that harbored a conditional Sox9 gene and a Villin-Cre transgene. RESULTS: In the absence of SOX9, Paneth cells were not formed, but the differentiation of other intestinal epithelial cell types was unaffected. The lack of SOX9 also lead to crypt enlargement, to a marked increase in cell proliferation throughout the crypts, and to replacement of the Paneth cells by proliferating epithelial cells. CONCLUSIONS: We conclude that SOX9 is required for the differentiation of Paneth cells. Our results elucidate an essential step in the differentiation of gut epithelium.
Authors: Kelli L VanDussen; Alexis J Carulli; Theresa M Keeley; Sanjeevkumar R Patel; Brent J Puthoff; Scott T Magness; Ivy T Tran; Ivan Maillard; Christian Siebel; Åsa Kolterud; Ann S Grosse; Deborah L Gumucio; Stephen A Ernst; Yu-Hwai Tsai; Peter J Dempsey; Linda C Samuelson Journal: Development Date: 2011-12-21 Impact factor: 6.868
Authors: Denise Al Alam; Soula Danopoulos; Kathy Schall; Frederic G Sala; Dana Almohazey; G Esteban Fernandez; Senta Georgia; Mark R Frey; Henri R Ford; Tracy Grikscheit; Saverio Bellusci Journal: Am J Physiol Gastrointest Liver Physiol Date: 2015-02-26 Impact factor: 4.052
Authors: Débora Sinner; Jennifer J Kordich; Jason R Spence; Robert Opoka; Scott Rankin; Suh-Chin J Lin; Diva Jonatan; Aaron M Zorn; James M Wells Journal: Mol Cell Biol Date: 2007-09-17 Impact factor: 4.272