Evaluation of photothermal effects in cartilage using FT-IR spectroscopy.

Photothermal effects after laser irradiation of cartilage are investigated using an infrared focal plane array (IR-FPA) camera and a Fourier transform infrared (FT-IR) spectrometer. The IR-FPA camera records radiometric temperature profile, while the local laser heating is applied to the sample; whereas the FT-IR spectrometer analyzes absorption peaks of cartilage constituents. As the major effect of photothermal heating in cartilage is water evaporation, spectral changes because of dehydration between control and laser-irradiated cartilage are recorded by FT-IR spectrometer measurements. Additionally, another interest was the observation of the spectral changes from macromolecules such as collagen and proteoglycans because of phase transformation and/or conformational changes after laser irradiation. The methodology may be useful for quantitative investigation of the relationship between the clinically important phenomenon of accelerated stress relaxation and the kinetics of macromolecular denaturation.