Tatsuya Kin1, Gregory S Korbutt. 1. Surgical-Medical Research Institute, University of Alberta, Edmonton, AB, Canada.
Abstract
BACKGROUND: The aim of this study was to compare the functional maturation of neonatal porcine islet (NPI) grafts exposed to long-term hyperglycemia with those implanted under euglycemic conditions. METHODS: mice Neonatal porcine islets were transplanted under the left renal capsule of diabetic SCID mice (group H), or in diabetic SCID mice who were also implanted with 500 BALB/c islets under the right renal capsule (group N). On day 42, the right kidneys were removed in both groups. RESULTS: No animals in group H achieved euglycemia within 3 weeks after transplantation. Thus, these mice were exposed to long-term hyperglycemia. Mice in group N became euglycemic immediately after transplantation, however after removal of BALB/c grafts on day 42 they exhibited significantly higher blood glucose levels than in group H and showed glucose intolerance after glucose administration. Cellular insulin content of NPI grafts harvested on day 58 or 72 was significantly lower in group N mice compared to group H. CONCLUSIONS: These results suggest that tight control of glycemia reduces the functional maturation of NPI grafts.
BACKGROUND: The aim of this study was to compare the functional maturation of neonatal porcine islet (NPI) grafts exposed to long-term hyperglycemia with those implanted under euglycemic conditions. METHODS:mice Neonatal porcine islets were transplanted under the left renal capsule of diabetic SCIDmice (group H), or in diabetic SCIDmice who were also implanted with 500 BALB/c islets under the right renal capsule (group N). On day 42, the right kidneys were removed in both groups. RESULTS: No animals in group H achieved euglycemia within 3 weeks after transplantation. Thus, these mice were exposed to long-term hyperglycemia. Mice in group N became euglycemic immediately after transplantation, however after removal of BALB/c grafts on day 42 they exhibited significantly higher blood glucose levels than in group H and showed glucose intolerance after glucose administration. Cellular insulin content of NPI grafts harvested on day 58 or 72 was significantly lower in group N mice compared to group H. CONCLUSIONS: These results suggest that tight control of glycemia reduces the functional maturation of NPI grafts.
Authors: R Valdes-Gonzalez; A L Rodriguez-Ventura; D J G White; E Bracho-Blanchet; A Castillo; B Ramírez-González; M G López-Santos; B H León-Mancilla; L M Dorantes Journal: Clin Exp Immunol Date: 2010-10-21 Impact factor: 4.330