BACKGROUND: Transmission of HIV-1 variants with resistance to reverse transcriptase (RT) and protease inhibitors has been widely characterized in developed countries. However, no clear evidence of primary resistance to HIV-1 fusion inhibitors has been shown so far. We wished to investigate the possibility of genotypic resistance to enfuvirtide (T20) in a cohort of antiretroviral-naive, recently infected patients. METHODS: We included patients from the Aquitaine Cohort with an estimated date of seroconversion in 2004 and 2005, a plasma sample obtained less than 18 months after seroconversion and no prior history of antiretroviral therapy. RT, protease and gp41 sequences were determined by direct population sequencing from plasma samples and drug resistance mutations were reported. RESULTS: A total of 55 patients were included in the study. The overall prevalence of transmitted HIV-1 resistance was 20%. Two patients had viruses with resistance mutations to T20. The first case had an N42D mutation in the HR1 region of the gp41, along with transmitted resistance mutations in the protease (D30N, M361, N88D) and in the RT (M41L, L210W, T215D). The second case had a G36D HR1 mutation, with no evidence of other drug resistance mutations. CONCLUSION: We have shown the first cases of primary resistance to T20 in recently infected patients in southwestern France. Epidemiological surveillance of the transmission of drug-resistant HIV-1 should include the resistance to T20.
BACKGROUND: Transmission of HIV-1 variants with resistance to reverse transcriptase (RT) and protease inhibitors has been widely characterized in developed countries. However, no clear evidence of primary resistance to HIV-1 fusion inhibitors has been shown so far. We wished to investigate the possibility of genotypic resistance to enfuvirtide (T20) in a cohort of antiretroviral-naive, recently infected patients. METHODS: We included patients from the Aquitaine Cohort with an estimated date of seroconversion in 2004 and 2005, a plasma sample obtained less than 18 months after seroconversion and no prior history of antiretroviral therapy. RT, protease and gp41 sequences were determined by direct population sequencing from plasma samples and drug resistance mutations were reported. RESULTS: A total of 55 patients were included in the study. The overall prevalence of transmitted HIV-1 resistance was 20%. Two patients had viruses with resistance mutations to T20. The first case had an N42D mutation in the HR1 region of the gp41, along with transmitted resistance mutations in the protease (D30N, M361, N88D) and in the RT (M41L, L210W, T215D). The second case had a G36D HR1 mutation, with no evidence of other drug resistance mutations. CONCLUSION: We have shown the first cases of primary resistance to T20 in recently infected patients in southwestern France. Epidemiological surveillance of the transmission of drug-resistant HIV-1 should include the resistance to T20.
Authors: Alexsander Augusto da Silveira; Ludimila Paula Vaz Cardoso; Roberta Barbosa Lopes Francisco; Mariane Martins de Araújo Stefani Journal: AIDS Res Hum Retroviruses Date: 2011-07-26 Impact factor: 2.205
Authors: Sarah E Hudelson; Natalia Marlowe; Wei Huang; Robert Bruce; Jessica D Church; Marla Husnik; Deborah Donnell; Thomas Coates; J Brooks Jackson; Margaret Chesney; Beryl Koblin; Susan H Eshleman Journal: AIDS Res Hum Retroviruses Date: 2009-07 Impact factor: 2.205
Authors: Alon Herschhorn; Andres Finzi; David M Jones; Joel R Courter; Akihiro Sugawara; Amos B Smith; Joseph G Sodroski Journal: PLoS One Date: 2011-11-01 Impact factor: 3.240
Authors: Joanna Smoleń-Dzirba; Magdalena Rosińska; Piotr Kruszyński; Jolanta Bratosiewicz-Wąsik; Robert Wojtyczka; Janusz Janiec; Bartosz Szetela; Marek Beniowski; Monika Bociąga-Jasik; Elżbieta Jabłonowska; Tomasz J Wąsik; And The Cascade Collaboration In EuroCoord Journal: Med Sci Monit Date: 2017-02-07