BACKGROUND: Galectin-1 (gal-1) and galectin-3 (gal-3), which are members of the mammalian beta-galactoside-binding proteins, recognise preferentially (Galbeta1-4GlcNAc) sequences of several cell surface oligosaccharides. In addition, gal-1 also binds to the Thomsen-Friedenreich (TF) antigen (Galbeta1-3GalNAc-). MATERIALS AND METHODS: Slides of frozen and paraffin-embedded placental tissue of patients with fetal intrauterine growth retardation (IUGR), preeclampsia, haemolysis, elevated liver enzymes, low platelets (HELLP) and normal term placentas were incubated with monoclonal and polyclonal antibodies against gal-1, gal-3 and TF. Staining reaction was performed with the avidin-biotinylated peroxidase complex (ABC) reagent. The intensity of the immunohistochemical reaction on the slides was analysed using a semi-quantitative score. The identity of galectin-expressing cells was analysed by using a double immunofluorescence method. RESULTS: We demonstrated immunohistochemically that the expression of gal-1 and gal-3 on the extravillous trophoblast (EVT) is significantly up-regulated in preeclamptic and HELLP placentas and unchanged compared with normal controls in IUGR placentas. The expression of the TF antigen is significantly up-regulated in IUGR and preeclamptic extravillous trophoblast cells and unchanged in HELLP placentas compared with normal controls. In addition, the expression of gal-1 is significantly up-regulated in the decidual tissue of preeclamptic placentas and in the villous trophoblast tissue of HELLP placentas. CONCLUSION: Our data showed that gal-1, gal-3 and TF were up-regulated on the membrane of EVT in preeclamptic placentas. In addition, the expression of gal-1 is significantly up-regulated in decidual tissue of preeclamptic placentas and villous trophoblast tissue of HELLP placentas. Taking into consideration the results of this study, we speculate that expression of both galectins and TF on the membrane of preeclamptic EVT and up-regulation of gal-1 in preeclamptic decidual cells may at least in part compensate for the apoptotic effects of maternal immune cells.
BACKGROUND:Galectin-1 (gal-1) and galectin-3 (gal-3), which are members of the mammalian beta-galactoside-binding proteins, recognise preferentially (Galbeta1-4GlcNAc) sequences of several cell surface oligosaccharides. In addition, gal-1 also binds to the Thomsen-Friedenreich (TF) antigen (Galbeta1-3GalNAc-). MATERIALS AND METHODS: Slides of frozen and paraffin-embedded placental tissue of patients with fetal intrauterine growth retardation (IUGR), preeclampsia, haemolysis, elevated liver enzymes, low platelets (HELLP) and normal term placentas were incubated with monoclonal and polyclonal antibodies against gal-1, gal-3 and TF. Staining reaction was performed with the avidin-biotinylated peroxidase complex (ABC) reagent. The intensity of the immunohistochemical reaction on the slides was analysed using a semi-quantitative score. The identity of galectin-expressing cells was analysed by using a double immunofluorescence method. RESULTS: We demonstrated immunohistochemically that the expression of gal-1 and gal-3 on the extravillous trophoblast (EVT) is significantly up-regulated in preeclamptic and HELLP placentas and unchanged compared with normal controls in IUGR placentas. The expression of the TF antigen is significantly up-regulated in IUGR and preeclamptic extravillous trophoblast cells and unchanged in HELLP placentas compared with normal controls. In addition, the expression of gal-1 is significantly up-regulated in the decidual tissue of preeclamptic placentas and in the villous trophoblast tissue of HELLP placentas. CONCLUSION: Our data showed that gal-1, gal-3 and TF were up-regulated on the membrane of EVT in preeclamptic placentas. In addition, the expression of gal-1 is significantly up-regulated in decidual tissue of preeclamptic placentas and villous trophoblast tissue of HELLP placentas. Taking into consideration the results of this study, we speculate that expression of both galectins and TF on the membrane of preeclamptic EVT and up-regulation of gal-1 in preeclamptic decidual cells may at least in part compensate for the apoptotic effects of maternal immune cells.
Authors: Nandor Gabor Than; Roberto Romero; Morris Goodman; Amy Weckle; Jun Xing; Zhong Dong; Yi Xu; Federica Tarquini; Andras Szilagyi; Peter Gal; Zhuocheng Hou; Adi L Tarca; Chong Jai Kim; Jung-Sun Kim; Saied Haidarian; Monica Uddin; Hans Bohn; Kurt Benirschke; Joaquin Santolaya-Forgas; Lawrence I Grossman; Offer Erez; Sonia S Hassan; Peter Zavodszky; Zoltan Papp; Derek E Wildman Journal: Proc Natl Acad Sci U S A Date: 2009-06-02 Impact factor: 11.205
Authors: Maja Weber; Claudia Göhner; Sebastian San Martin; Aurelia Vattai; Stefan Hutter; Mario Parraga; Udo Jeschke; Ekkehard Schleussner; Udo R Markert; Justine S Fitzgerald Journal: Cell Adh Migr Date: 2016-02-25 Impact factor: 3.405
Authors: Mirian Mendoza; Dongli Lu; Angela Ballesteros; Sandra M Blois; Kelsey Abernathy; Chiguang Feng; Charles J Dimitroff; Jonathan Zmuda; Maria Panico; Anne Dell; Gerardo R Vasta; Stuart M Haslam; Gabriela Dveksler Journal: Glycobiology Date: 2020-10-21 Impact factor: 4.313
Authors: Nandor Gabor Than; Roberto Romero; Offer Erez; Amy Weckle; Adi L Tarca; John Hotra; Asad Abbas; Yu Mi Han; Sung-Su Kim; Juan Pedro Kusanovic; Francesca Gotsch; Zhuocheng Hou; Joaquin Santolaya-Forgas; Kurt Benirschke; Zoltan Papp; Lawrence I Grossman; Morris Goodman; Derek E Wildman Journal: Proc Natl Acad Sci U S A Date: 2008-09-29 Impact factor: 11.205
Authors: Nandor Gabor Than; Sung-Su Kim; Asad Abbas; Yu Mi Han; John Hotra; Adi L Tarca; Offer Erez; Derek E Wildman; Juan Pedro Kusanovic; Beth Pineles; Daniel Montenegro; Samuel S Edwin; Shali Mazaki-Tovi; Francesca Gotsch; Jimmy Espinoza; Sonia S Hassan; Zoltan Papp; Roberto Romero Journal: Am J Reprod Immunol Date: 2008-10 Impact factor: 3.886
Authors: Nandor Gabor Than; Offer Erez; Derek E Wildman; Adi L Tarca; Samuel S Edwin; Asad Abbas; John Hotra; Juan Pedro Kusanovic; Francesca Gotsch; Sonia S Hassan; Jimmy Espinoza; Zoltan Papp; Roberto Romero Journal: J Matern Fetal Neonatal Med Date: 2008-07