Literature DB >> 17661025

Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003.

Laxmi Prasad Thapa1, Tae-Jin Oh, Hei Chan Lee, Kwangkyoung Liou, Je Won Park, Yeo Joon Yoon, Jae Kyung Sohng.   

Abstract

The pSKC2 cosmid, which has 32 kb and 28 open-reading frames, was isolated from Streptomyces kanamyceticus ATCC12853 as the gene cluster of kanamycin. This gene cluster includes the minimal biosynthetic genes of kanamycin with the resistance and regulatory genes. It was heterologously expressed in Streptomyces venezuelae YJ003, which has the advantage of fast growth, good efficiency of the transformation host, and rapid production of the aminoglycosides antibiotic. The isolated compound was analyzed by electrospray ionization-mass spectrometry, liquid chromatography-mass spectrometry, high-performance liquid chromatography, and tandem mass spectrometry and shows a molecular weight of 485 as kanamycin A.

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Year:  2007        PMID： 17661025     DOI： 10.1007/s00253-007-1096-4

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  10 in total

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Authors:  Taylor B Cook; Brian F Pfleger
Journal:  Medchemcomm       Date:  2019-04-25       Impact factor: 3.597

Review 2.  Streptomycetes: Surrogate hosts for the genetic manipulation of biosynthetic gene clusters and production of natural products.

Authors:  Keshav K Nepal; Guojun Wang
Journal:  Biotechnol Adv       Date:  2018-10-09       Impact factor: 14.227

3.  Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.

Authors:  Je Won Park; Sung Ryeol Park; Keshav Kumar Nepal; Ah Reum Han; Yeon Hee Ban; Young Ji Yoo; Eun Ji Kim; Eui Min Kim; Dooil Kim; Jae Kyung Sohng; Yeo Joon Yoon
Journal:  Nat Chem Biol       Date:  2011-10-09       Impact factor: 15.040

Review 4.  Progress in aminocyclitol biosynthesis.

Authors:  Taifo Mahmud
Journal:  Curr Opin Chem Biol       Date:  2009-03-25       Impact factor: 8.822

5.  Biosynthetic gene cluster of cetoniacytone A, an unusual aminocyclitol from the endosymbiotic Bacterium Actinomyces sp. Lu 9419.

Authors:  Xiumei Wu; Patricia M Flatt; Hui Xu; Taifo Mahmud
Journal:  Chembiochem       Date:  2009-01-26       Impact factor: 3.164

6.  Enhanced heterologous production of desosaminyl macrolides and their hydroxylated derivatives by overexpression of the pikD regulatory gene in Streptomyces venezuelae.

Authors:  Won Seok Jung; Soon Jeong Jeong; Sung Ryeol Park; Cha Yong Choi; Byoung Chul Park; Je Won Park; Yeo Joon Yoon
Journal:  Appl Environ Microbiol       Date:  2008-02-01       Impact factor: 4.792

7.  A system for the targeted amplification of bacterial gene clusters multiplies antibiotic yield in Streptomyces coelicolor.

Authors:  Takeshi Murakami; Jan Burian; Koji Yanai; Mervyn J Bibb; Charles J Thompson
Journal:  Proc Natl Acad Sci U S A       Date:  2011-09-08       Impact factor: 11.205

Review 8.  An insight into the "-omics" based engineering of streptomycetes for secondary metabolite overproduction.

Authors:  Amit Kumar Chaudhary; Dipesh Dhakal; Jae Kyung Sohng
Journal:  Biomed Res Int       Date:  2013-09-02       Impact factor: 3.411

Review 9.  Cloning and Heterologous Expression of a Large-sized Natural Product Biosynthetic Gene Cluster in Streptomyces Species.

Authors:  Hee-Ju Nah; Hye-Rim Pyeon; Seung-Hoon Kang; Si-Sun Choi; Eung-Soo Kim
Journal:  Front Microbiol       Date:  2017-03-15       Impact factor: 5.640

10.  Establishment of a highly efficient conjugation protocol for Streptomyces kanamyceticus ATCC12853.

Authors:  Shuman Zhang; Tiansheng Chen; Jia Jia; Liwen Guo; Huizheng Zhang; Chao Li; Renzhong Qiao
Journal:  Microbiologyopen       Date:  2018-11-17       Impact factor: 3.139
  10 in total

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