MOTIVATION: The genome of the social amoeba Dictyostelium discoideum contains an unusually large number of polyketide synthase (PKS) genes. An analysis of the genes is a first step towards understanding the biological roles of their products and exploiting novel products. RESULTS: A total of 45 Type I iterative PKS genes were found, 5 of which are probably pseudogenes. Catalytic domains that are homologous with known PKS sequences as well as possible novel domains were identified. The genes often occurred in clusters of 2-5 genes, where members of the cluster had very similar sequences. The D.discoideum PKS genes formed a clade distinct from fungal and bacterial genes. All nine genes examined by RT-PCR were expressed, although at different developmental stages. The promoters of PKS genes were much more divergent than the structural genes, although we have identified motifs that are unique to some PKS gene promoters.
MOTIVATION: The genome of the social amoeba Dictyostelium discoideum contains an unusually large number of polyketide synthase (PKS) genes. An analysis of the genes is a first step towards understanding the biological roles of their products and exploiting novel products. RESULTS: A total of 45 Type I iterative PKS genes were found, 5 of which are probably pseudogenes. Catalytic domains that are homologous with known PKS sequences as well as possible novel domains were identified. The genes often occurred in clusters of 2-5 genes, where members of the cluster had very similar sequences. The D.discoideum PKS genes formed a clade distinct from fungal and bacterial genes. All nine genes examined by RT-PCR were expressed, although at different developmental stages. The promoters of PKS genes were much more divergent than the structural genes, although we have identified motifs that are unique to some PKS gene promoters.
Authors: Andrew J Heidel; Hajara M Lawal; Marius Felder; Christina Schilde; Nicholas R Helps; Budi Tunggal; Francisco Rivero; Uwe John; Michael Schleicher; Ludwig Eichinger; Matthias Platzer; Angelika A Noegel; Pauline Schaap; Gernot Glöckner Journal: Genome Res Date: 2011-07-14 Impact factor: 9.043
Authors: Richard Sucgang; Alan Kuo; Xiangjun Tian; William Salerno; Anup Parikh; Christa L Feasley; Eileen Dalin; Hank Tu; Eryong Huang; Kerrie Barry; Erika Lindquist; Harris Shapiro; David Bruce; Jeremy Schmutz; Asaf Salamov; Petra Fey; Pascale Gaudet; Christophe Anjard; M Madan Babu; Siddhartha Basu; Yulia Bushmanova; Hanke van der Wel; Mariko Katoh-Kurasawa; Christopher Dinh; Pedro M Coutinho; Tamao Saito; Marek Elias; Pauline Schaap; Robert R Kay; Bernard Henrissat; Ludwig Eichinger; Francisco Rivero; Nicholas H Putnam; Christopher M West; William F Loomis; Rex L Chisholm; Gad Shaulsky; Joan E Strassmann; David C Queller; Adam Kuspa; Igor V Grigoriev Journal: Genome Biol Date: 2011-02-28 Impact factor: 13.583
Authors: Antonio Starcevic; Jurica Zucko; Jurica Simunkovic; Paul F Long; John Cullum; Daslav Hranueli Journal: Nucleic Acids Res Date: 2008-10-31 Impact factor: 16.971