AIM: To investigate whether anti-H pylori antibodies have cross-reaction with antigens of erythrocyte membrane. METHODS: Blood samples were collected from 14 volunteers (8 positive and 6 negative for H pylori detected by (13)C-urea breath test) of the general population. Erythrocyte membrane proteins of the subjects were examined by Western blot using anti-H pylori serum. The proteins related to the positive bands were identified by mass spectrum analysis. RESULTS: Anti-H pylori antibodies had cross-reaction with the proteins of about 50 kDa of erythrocyte membranes in all samples independent of H pylori infection. One protein in the positive band was identified as Chain S, the crystal structure of the cytoplasmic domain of human erythrocyte Band-3 protein. CONCLUSION: Anti-H pylori antibodies cross-react with some antigens of human erythrocyte membrane, which may provide a clue for the relationship between H pylori infection and vascular disorders.
AIM: To investigate whether anti-H pylori antibodies have cross-reaction with antigens of erythrocyte membrane. METHODS: Blood samples were collected from 14 volunteers (8 positive and 6 negative for H pylori detected by (13)C-urea breath test) of the general population. Erythrocyte membrane proteins of the subjects were examined by Western blot using anti-H pylori serum. The proteins related to the positive bands were identified by mass spectrum analysis. RESULTS: Anti-H pylori antibodies had cross-reaction with the proteins of about 50 kDa of erythrocyte membranes in all samples independent of H pylori infection. One protein in the positive band was identified as Chain S, the crystal structure of the cytoplasmic domain of human erythrocyte Band-3 protein. CONCLUSION: Anti-H pylori antibodies cross-react with some antigens of human erythrocyte membrane, which may provide a clue for the relationship between H pylori infection and vascular disorders.
Authors: Antonio Pietroiusti; Marina Diomedi; Mauro Silvestrini; Letizia Maria Cupini; Ida Luzzi; Maria Jesus Gomez-Miguel; Antonio Bergamaschi; Andrea Magrini; Tiziana Carrabs; Marina Vellini; Alberto Galante Journal: Circulation Date: 2002-07-30 Impact factor: 29.690
Authors: Francesco Franceschi; Antonia R Sepulveda; Antonio Gasbarrini; Paolo Pola; Nicolò Gentiloni Silveri; Giovanni Gasbarrini; David Y Graham; Robert M Genta Journal: Circulation Date: 2002-07-23 Impact factor: 29.690
Authors: Agha W Haider; Peter W F Wilson; Martin G Larson; Jane C Evans; Eric L Michelson; Philip A Wolf; Christopher J O'Donnell; Daniel Levy Journal: J Am Coll Cardiol Date: 2002-10-16 Impact factor: 24.094
Authors: A J Grau; F Buggle; S Heindl; C Steichen-Wiehn; T Banerjee; M Maiwald; M Rohlfs; H Suhr; W Fiehn; H Becher Journal: Stroke Date: 1995-03 Impact factor: 7.914