R Goethe1, T Basler, L Phi-van. 1. Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine, Bischofsholer Damm 15, 30173 Hannover, Germany.
Abstract
OBJECTIVE: The transcription factor CCAAT/enhancer- binding protein (C/EBP) beta is involved in inflammatory responses in immune cells, including myelomonocytic cells. In this study, signal transduction pathways regulating C/EBPbeta expression were investigated. METHODS: The expression of C/EBPbeta mRNA in cells treated with various activators and inhibitors of PKA and PKC was analyzed by Northern blot hybridization. C/EBPbeta promoter activity was investigated by transient transfection assays with C/EBPbeta promoter CAT constructs. RESULTS: Phorbol 12-myristate 13-acetate (PMA), forskolin and 3-isobutyl-1-methyl-xanthine (IBMX), an inhibitor of cAMP and cGMP phosphodiesterases, but not cGMP, when added to chicken myelomonocytic HD11 cells, markedly stimulated the C/EBPbeta mRNA expression. However, transfection experiments using HD11 cells showed that CAT constructs controlled by the 5' flanking sequence from -704 to +24 of chicken C/EBPbeta gene were activated by PMA, but not by forskolin. In contrast to forskolin, IBMX was able to activate the C/EBPbeta promoter CAT constructs. Further transient transfection experiments using other cell lines demonstrated that the chicken C/EBPbeta promoter was responsive to forskolin in mouse fibroblasts NIH3T3, but not in human hepatoma HepG2 cells. Increase in C/EBPbeta mRNA stability in HD11 cells was induced by forskolin and PMA. CONCLUSION: The results indicate that the C/EBPbeta gene is regulated transcriptionally as well as post-transcriptionally in response to forskolin and PMA, and the forskolin responsiveness of the C/EBPbeta promoter seems to depend on cellular cAMP turnover.
OBJECTIVE: The transcription factor CCAAT/enhancer- binding protein (C/EBP) beta is involved in inflammatory responses in immune cells, including myelomonocytic cells. In this study, signal transduction pathways regulating C/EBPbeta expression were investigated. METHODS: The expression of C/EBPbeta mRNA in cells treated with various activators and inhibitors of PKA and PKC was analyzed by Northern blot hybridization. C/EBPbeta promoter activity was investigated by transient transfection assays with C/EBPbeta promoter CAT constructs. RESULTS:Phorbol 12-myristate 13-acetate (PMA), forskolin and 3-isobutyl-1-methyl-xanthine (IBMX), an inhibitor of cAMP and cGMP phosphodiesterases, but not cGMP, when added to chicken myelomonocytic HD11 cells, markedly stimulated the C/EBPbeta mRNA expression. However, transfection experiments using HD11 cells showed that CAT constructs controlled by the 5' flanking sequence from -704 to +24 of chickenC/EBPbeta gene were activated by PMA, but not by forskolin. In contrast to forskolin, IBMX was able to activate the C/EBPbeta promoter CAT constructs. Further transient transfection experiments using other cell lines demonstrated that the chickenC/EBPbeta promoter was responsive to forskolin in mouse fibroblasts NIH3T3, but not in humanhepatoma HepG2 cells. Increase in C/EBPbeta mRNA stability in HD11 cells was induced by forskolin and PMA. CONCLUSION: The results indicate that the C/EBPbeta gene is regulated transcriptionally as well as post-transcriptionally in response to forskolin and PMA, and the forskolin responsiveness of the C/EBPbeta promoter seems to depend on cellular cAMP turnover.
Authors: Lisa C Loram; Frederick R Taylor; Keith A Strand; Jacqueline A Harrison; Rachael Rzasalynn; Paige Sholar; Jayson Rieger; Steven F Maier; Linda R Watkins Journal: Brain Behav Immun Date: 2013-06-28 Impact factor: 7.217
Authors: Marta Podgórska; Monika Ołdak; Anna Marthaler; Alina Fingerle; Barbara Walch-Rückheim; Stefan Lohse; Cornelia S L Müller; Thomas Vogt; Mart Ustav; Artur Wnorowski; Magdalena Malejczyk; Sławomir Majewski; Sigrun Smola Journal: Front Microbiol Date: 2018-03-07 Impact factor: 5.640