Literature DB >> 17656746

Microwave-assisted processing and embedding for transmission electron microscopy.

Paul Webster1.   

Abstract

Microwave processors can provide a means of rapid processing and resin embedding for biological specimens that are to be sectioned and examined by transmission electron microscopy. This chapter describes a microwave-assisted protocol for processing, dehydrating, and embedding biological material, from living specimens to blocks embedded in sectionable resin in 4 h or less.

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Year:  2007        PMID： 17656746     DOI： 10.1007/978-1-59745-294-6_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Rapid specimen preparation to improve the throughput of electron microscopic volume imaging for three-dimensional analyses of subcellular ultrastructures with serial block-face scanning electron microscopy.

Authors:  Truc Quynh Thai; Huy Bang Nguyen; Sei Saitoh; Bao Wu; Yurika Saitoh; Satoshi Shimo; Yaser Hosny Ali Elewa; Osamu Ichii; Yasuhiro Kon; Takashi Takaki; Kensuke Joh; Nobuhiko Ohno
Journal:  Med Mol Morphol       Date:  2016-02-11       Impact factor: 2.309

2.  Intervacuolar transport and unique topology of GRA14, a novel dense granule protein in Toxoplasma gondii.

Authors:  Michael E Rome; Josh R Beck; Jay M Turetzky; Paul Webster; Peter J Bradley
Journal:  Infect Immun       Date:  2008-09-02       Impact factor: 3.441

3.  Rapid microwave fixation of cell monolayers preserves microtubule-associated cell structures.

Authors:  Siegfried Reipert; Harald Kotisch; Bhuma Wysoudil; Gerhard Wiche
Journal:  J Histochem Cytochem       Date:  2008-04-14       Impact factor: 2.479

4.  Towards native-state imaging in biological context in the electron microscope.

Authors:  Anne E Weston; Hannah E J Armer; Lucy M Collinson
Journal:  J Chem Biol       Date:  2009-11-15

Review 5.  Modern uses of electron microscopy for detection of viruses.

Authors:  Cynthia S Goldsmith; Sara E Miller
Journal:  Clin Microbiol Rev       Date:  2009-10       Impact factor: 26.132

6.  High-contrast en bloc staining of neuronal tissue for field emission scanning electron microscopy.

Authors:  Juan Carlos Tapia; Narayanan Kasthuri; Kenneth J Hayworth; Richard Schalek; Jeff W Lichtman; Stephen J Smith; JoAnn Buchanan
Journal:  Nat Protoc       Date:  2012-01-12       Impact factor: 13.491

7.  Knockdown of p180 eliminates the terminal differentiation of a secretory cell line.

Authors:  Payam Benyamini; Paul Webster; David I Meyer
Journal:  Mol Biol Cell       Date:  2008-11-26       Impact factor: 4.138

8.  Glucocorticoid-stimulated, transcription-independent release of annexin A1 by cochlear Hensen cells.

Authors:  F Kalinec; P Webster; A Maricle; D Guerrero; D N Chakravarti; B Chakravarti; R Gellibolian; G Kalinec
Journal:  Br J Pharmacol       Date:  2009-12       Impact factor: 8.739

9.  Impaired autophagic flux mediates acinar cell vacuole formation and trypsinogen activation in rodent models of acute pancreatitis.

Authors:  Olga A Mareninova; Kip Hermann; Samuel W French; Mark S O'Konski; Stephen J Pandol; Paul Webster; Ann H Erickson; Nobuhiko Katunuma; Fred S Gorelick; Ilya Gukovsky; Anna S Gukovskaya
Journal:  J Clin Invest       Date:  2009-10-01       Impact factor: 14.808

10.  Engineered protein nano-compartments for targeted enzyme localization.

Authors:  Swati Choudhary; Maureen B Quin; Mark A Sanders; Ethan T Johnson; Claudia Schmidt-Dannert
Journal:  PLoS One       Date:  2012-03-12       Impact factor: 3.240
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