| Literature DB >> 17656743 |
Abstract
This chapter covers the conventional methods and considerations for preparing cultured cells for examination in the transmission electron microscope. Techniques for handling cells grown in liquid culture, as well as on substrates such as culture dishes or agar, are discussed. Directions are given on how to prepare the most commonly used buffers, fixatives, enrobement media, and embedding resins. These methods may be applied to most cultured organisms, from bacteria to mammalian cells.Entities:
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Year: 2007 PMID： 17656743 DOI： 10.1007/978-1-59745-294-6_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745