| Literature DB >> 17655961 |
Harald J Ruijssenaars1, Eric M G M Sperling, Peter H G Wiegerinck, Ferry T L Brands, Jan Wery, Jan A M de Bont.
Abstract
A steroid 15beta-hydroxylating whole-cell solvent tolerant biocatalyst was constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12. Testosterone hydroxylation was improved by a factor 16 by co-expressing Fer, a putative Fe-S protein from Bacillus subtilis. In addition, the specificity for 15beta-hydroxylation was improved by mutating threonine residue 248 of CYP106A2 into valine. These new insights provide the basis for an optimized whole-cell steroid-hydroxylating biocatalyst that can be applied with an organic solvent phase.Entities:
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Year: 2007 PMID： 17655961 DOI： 10.1016/j.jbiotec.2007.06.007
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307