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Literature DB >> 17655960

Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16.

Diana Dinu¹, Marina Tamara Nechifor, Gheorghe Stoian, Marieta Costache, Anca Dinischiotu.

Abstract

A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were analyzed. The purified enzymes were classified with respect to their biochemical properties and substrate specificity. Among these proteins, one revealed polygalacturonase activity, another appeared to be a pectin methylesterase and three were identified as pectate lyases. The capacity of the fungus A. niger to produce pectate lyases with optimum pH in acidic domain was reported for the first time.

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Year: 2007 PMID： 17655960 DOI： 10.1016/j.jbiotec.2007.06.005

Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307

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Cited

9 in total

1. Purification and characterization of the exopolygalacturonase produced by Aspergillus giganteus in submerged cultures.

Authors: Danielle Biscaro Pedrolli; Eleonora Cano Carmona
Journal: J Ind Microbiol Biotechnol Date: 2010-03-04 Impact factor: 3.346

2. Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice.

Authors: Gautam Anand; Sangeeta Yadav; Dinesh Yadav
Journal: 3 Biotech Date: 2017-05-31 Impact factor: 2.406

3. Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025.

Authors: Akhilesh Thakur; Roma Pahwa; Smarika Singh; Reena Gupta
Journal: Enzyme Res Date: 2010-06-01

4. Quality Attributes of Cupuaçu Juice in Response to Treatment with Crude Enzyme Extract Produced by Aspergillus japonicus 586.

Authors: Maria Francisca Simas Teixeira; Jerusa Souza Andrade; Ormezinda Celeste Cristo Fernandes; Nelson Durán; José Luiz de Lima Filho
Journal: Enzyme Res Date: 2011-11-01

Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16.

1. Purification and characterization of the exopolygalacturonase produced by Aspergillus giganteus in submerged cultures.

2. Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice.

3. Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025.

4. Quality Attributes of Cupuaçu Juice in Response to Treatment with Crude Enzyme Extract Produced by Aspergillus japonicus 586.

5. Enhanced production of pectinase by Aspergillus terreus NCFT 4269.10 using banana peels as substrate.

6. Isolation of Polygalacturonase-Producing Bacterial Strain from Tomatoes (Lycopersicon esculentum Mill.).

7. Partial Purification and Characterization of Exo-Polygalacturonase Produced by Penicillium oxalicum AUMC 4153.

8. Improvement on Thermostability of Pectate Lyase and Its Potential Application to Ramie Degumming.

9. Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges.