Chun-Lei Wang1, Jian Kang, Zhen-Hua Li. 1. Institute of Respiratory Disease, First Affiliated Hospital of China Medical University, Shenyang 110001, China.
Abstract
OBJECTIVE: To investigate the phosphorylation of the proteins, NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in neutrophil cell membranes from bronchoalveolar lavage fluid (BALF) and peripheral blood of patients with idiopathic interstitial fibrosis (IPF). METHODS: Fifteen patients with IPF (IPF group) and 7 controls without lung diseases (control group) were included. The superoxide anion of peripheral blood neutrophils were measured using the cytochrome C reducing method. Immunocytochemical method and Western blotting were used to measure the phosphorylation expression of cell protein in BALF and phosphorylation of membrane protein and p47(-PHOX) and p67(-PHOX) factor in peripheral blood neutrophils, respectively. RESULTS: The phosphorylation of BALF neutrophils (average absorbance) was 0.14 +/- 0.01 in the control group, 0.19 +/- 0.02 in the IPF group, the difference being significant. The phosphorylation levels of relative molecular weight 80,000, 58,000, and 45,000 membrane proteins and p47(-PHOX) and p67(-PHOX) of the peripheral blood neutrophils in the IPF group were higher than those in the control group. The level of superoxide anion in the peripheral blood neutrophils of the IPF group was negatively correlated with forced vital capacity, one second forced expiratory volume, PaO2, diffusing capacity of the lung for carbon monoxide. CONCLUSION: The level of superoxide anion of the blood neutrophils is associated with pulmonary fibrosis. There is an increased expression of NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in IPF patients.
OBJECTIVE: To investigate the phosphorylation of the proteins, NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in neutrophil cell membranes from bronchoalveolar lavage fluid (BALF) and peripheral blood of patients with idiopathic interstitial fibrosis (IPF). METHODS: Fifteen patients with IPF (IPF group) and 7 controls without lung diseases (control group) were included. The superoxide anion of peripheral blood neutrophils were measured using the cytochrome C reducing method. Immunocytochemical method and Western blotting were used to measure the phosphorylation expression of cell protein in BALF and phosphorylation of membrane protein and p47(-PHOX) and p67(-PHOX) factor in peripheral blood neutrophils, respectively. RESULTS: The phosphorylation of BALF neutrophils (average absorbance) was 0.14 +/- 0.01 in the control group, 0.19 +/- 0.02 in the IPF group, the difference being significant. The phosphorylation levels of relative molecular weight 80,000, 58,000, and 45,000 membrane proteins and p47(-PHOX) and p67(-PHOX) of the peripheral blood neutrophils in the IPF group were higher than those in the control group. The level of superoxide anion in the peripheral blood neutrophils of the IPF group was negatively correlated with forced vital capacity, one second forced expiratory volume, PaO2, diffusing capacity of the lung for carbon monoxide. CONCLUSION: The level of superoxide anion of the blood neutrophils is associated with pulmonary fibrosis. There is an increased expression of NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in IPFpatients.
Authors: Carmen Veith; Agnes W Boots; Musa Idris; Frederik-Jan van Schooten; Albert van der Vliet Journal: Antioxid Redox Signal Date: 2019-04-05 Impact factor: 8.401
Authors: Srikanth Pendyala; Peter V Usatyuk; Irina A Gorshkova; Joe G N Garcia; Viswanathan Natarajan Journal: Antioxid Redox Signal Date: 2009-04 Impact factor: 8.401