Literature DB >> 1764972

Experimental and theoretical investigations on the validity of the geometrical optics model for calculating the stability of optical traps.

T C Schut1, G Hesselink, B G de Grooth, J Greve.   

Abstract

We have developed a computer program based on the geometrical optics approach proposed by Roosen to calculate the forces on dielectric spheres in focused laser beams. We have explicitly taken into account the polarization of the laser light and thd divergence of the laser beam. The model can be used to evaluate the stability of optical traps in a variety of different optical configurations. Our calculations explain the experimental observation by Ashkin that a stable single-beam optical trap, without the help of the gravitation force, can be obtained with a strongly divergent laser beam. Our calculations also predict a different trap stability in the directions orthogonal and parallel to the polarization direction of the incident light. Different experimental methods were used to test the predictions of the model for the gravity trap. A new method for measuring the radiation force along the beam axis in both the stable and instable regions is presented. Measurements of the radiation force on polystyrene spheres with diameters of 7.5 and 32 microns in a TEM00-mode laser beam showed a good qualitative correlation with the predictions and a slight quantitative difference. The validity of the geometrical approximations involved in the model will be discussed for spheres of different sizes and refractive indices.

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Year:  1991        PMID: 1764972     DOI: 10.1002/cyto.990120603

Source DB:  PubMed          Journal:  Cytometry        ISSN: 0196-4763


  2 in total

1.  Depth-resolved measurement of optical radiation-pressure forces with optical coherence tomography.

Authors:  Nichaluk Leartprapun; Rishyashring R Iyer; Steven G Adie
Journal:  Opt Express       Date:  2018-02-05       Impact factor: 3.894

2.  Mechanical properties of neuronal growth cone membranes studied by tether formation with laser optical tweezers.

Authors:  J Dai; M P Sheetz
Journal:  Biophys J       Date:  1995-03       Impact factor: 4.033

  2 in total

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