The Use of an Improved Fluorescent Antibody Procedure in the Demonstration of Leptospira in Animal Tissues.

Increased sensitivity of the fluorescent antibody procedure was achieved by modification of the method employed. Specimens collected from experimentally infected laboratory animals and naturally infected domestic and wild animals were examined by means of this method. The results compared very favorably with those obtained by other investigators who used serological, cultural, animal inoculation and silver impregnation procedures on the same specimens.