Literature DB >> 1764505

Fast preparative separation of 'native' core E coli 30S ribosomal proteins.

C Cachia1, P J Flamion, J P Schreiber.   

Abstract

We have developed an ion-exchange high performance liquid chromatographic method for preparative separation of 'core' proteins from E coli 30S ribosomal subunits, extracted with salt under non-denaturing conditions. This method yields individual proteins in pure and native form at high concentrations, (5 to 25 mg/ml) suitable for direct use in 1D-, 2D- or 3D-NMR studies.

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Year:  1991        PMID： 1764505     DOI： 10.1016/0300-9084(91)90029-z

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  5 in total

1.  In vivo selection of functional ribosomes with variations in the rRNA-binding site of Escherichia coli ribosomal protein S8: evolutionary implications.

Authors:  H Moine; C L Squires; B Ehresmann; C Ehresmann
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-18       Impact factor: 11.205

2.  Ribosomal protein S15 from Escherichia coli modulates its own translation by trapping the ribosome on the mRNA initiation loading site.

Authors:  C Philippe; F Eyermann; L Bénard; C Portier; B Ehresmann; C Ehresmann
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-15       Impact factor: 11.205

3.  Structural elements of rps0 mRNA involved in the modulation of translational initiation and regulation of E. coli ribosomal protein S15.

Authors:  C Philippe; L Bénard; F Eyermann; C Cachia; S V Kirillov; C Portier; B Ehresmann; C Ehresmann
Journal:  Nucleic Acids Res       Date:  1994-07-11       Impact factor: 16.971

4.  Role of conserved nucleotides in building the 16S rRNA binding site of E. coli ribosomal protein S8.

Authors:  C Allmang; M Mougel; E Westhof; B Ehresmann; C Ehresmann
Journal:  Nucleic Acids Res       Date:  1994-09-11       Impact factor: 16.971

5.  Molecular dissection of the pseudoknot governing the translational regulation of Escherichia coli ribosomal protein S15.

Authors:  C Philippe; L Bénard; C Portier; E Westhof; B Ehresmann; C Ehresmann
Journal:  Nucleic Acids Res       Date:  1995-01-11       Impact factor: 16.971
  5 in total

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