Literature DB >> 17644646

Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts.

Kathryn M Miller1, Charles R Sterling.   

Abstract

Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.

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Year:  2007        PMID： 17644646      PMCID： PMC2074905          DOI： 10.1128/AEM.00668-07

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  11 in total

Review 1.  Biology of Giardia lamblia.

Authors:  R D Adam
Journal:  Clin Microbiol Rev       Date:  2001-07       Impact factor: 26.132

2.  Distribution of Giardia duodenalis genotypes and subgenotypes in raw urban wastewater in Milwaukee, Wisconsin.

Authors:  Irshad M Sulaiman; Jianlin Jiang; Ajaib Singh; Lihua Xiao
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792

3.  Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces.

Authors:  C F L Amar; P H Dear; S Pedraza-Díaz; N Looker; E Linnane; J McLauchlin
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

4.  Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCR.

Authors:  C Kaucner; T Stinear
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

5.  Species-specific, nested PCR-restriction fragment length polymorphism detection of single Cryptosporidium parvum oocysts.

Authors:  G D Sturbaum; C Reed; P J Hoover; B H Jost; M M Marshall; C R Sterling
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

6.  Comparison of two target genes for detection and genotyping of Giardia lamblia in human feces by PCR and PCR-restriction fragment length polymorphism.

Authors:  Isabelle Bertrand; Laetitia Albertini; Janine Schwartzbrod
Journal:  J Clin Microbiol       Date:  2005-12       Impact factor: 5.948

7.  Effects of stunting, diarrhoeal disease, and parasitic infection during infancy on cognition in late childhood: a follow-up study.

Authors:  Douglas S Berkman; Andres G Lescano; Robert H Gilman; Sonia L Lopez; Maureen M Black
Journal:  Lancet       Date:  2002-02-16       Impact factor: 79.321

Review 8.  Drinking water treatment processes for removal of Cryptosporidium and Giardia.

Authors:  Walter Q Betancourt; Joan B Rose
Journal:  Vet Parasitol       Date:  2004-12-09       Impact factor: 2.738

9.  Detection of viable Giardia cysts by amplification of heat shock-induced mRNA.

Authors:  M Abbaszadegan; M S Huber; C P Gerba; I L Pepper
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792

10.  Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage.

Authors:  Rebecca A Guy; Pierre Payment; Ulrich J Krull; Paul A Horgen
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792
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  5 in total

1.  Genotyping of Giardia duodenalis cysts by new real-time PCR assays for detection of mixed infections in human samples.

Authors:  Andre Almeida; Edoardo Pozio; Simone M Cacciò
Journal:  Appl Environ Microbiol       Date:  2010-01-15       Impact factor: 4.792

2.  Detection of Batrachochytrium dendrobatidis in endemic salamander species from central Texas.

Authors:  James P Gaertner; Michael R J Forstner; Lisa O'Donnell; Dittmar Hahn
Journal:  Ecohealth       Date:  2009-05-08       Impact factor: 3.184

3.  Distribution and diversity of nitrite-dependent anaerobic methane-oxidising bacteria in the sediments of the Qiantang River.

Authors:  Li-dong Shen; Shuai Liu; Qun Zhu; Xiao-yu Li; Chen Cai; Dong-qing Cheng; Li-ping Lou; Xiang-yang Xu; Ping Zheng; Bao-lan Hu
Journal:  Microb Ecol       Date:  2013-11-23       Impact factor: 4.552

4.  Allelic sequence heterozygosity in single Giardia parasites.

Authors:  Johan Ankarklev; Staffan G Svärd; Marianne Lebbad
Journal:  BMC Microbiol       Date:  2012-05-03       Impact factor: 3.605

5.  Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample.

Authors:  Tsui-Kang Hsu; Jung-Sheng Chen; Hsin-Chi Tsai; Chi-Wei Tao; Yu-Yin Yang; Ying-Chin Tseng; Yi-Jie Kuo; Dar-Der Ji; Jagat Rathod; Bing-Mu Hsu
Journal:  Sci Rep       Date:  2021-11-05       Impact factor: 4.379
  5 in total

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