| Literature DB >> 17643561 |
Valéria A Conforti1, David M de Avila, Nada S Cummings, Kenny J Wells, Hasan Ulker, Jerry J Reeves.
Abstract
A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum. Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization.Entities:
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Year: 2007 PMID： 17643561 DOI： 10.1016/j.vaccine.2007.05.056
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641