Maize NDC80 is a constitutive feature of the central kinetochore.

In yeast and animals, Nuclear Division Cycle 80 (NDC80) is an important kinetochore protein that binds to microtubules and mediates chromosome movement. Its localization pattern is unusual, since it is generally not viewed as either an inner (centromeric chromatin) or outer (regulatory) component of the kinetochore. Here we report the characterization of NDC80 in a higher plant. By taking advantage of the large meiotic kinetochores of maize, we were able to show that NDC80 localizes outside of the constitutive kinetochore protein CENP-C. Further, a detailed analysis of mitosis indicates that NDC80 is stably present on kinetochores throughout the cell cycle. The quantity of NDC80 positively correlates with measured quantities of DNA and CENP-C, suggesting that NDC80 rapidly associates with DNA following replication and is stably maintained at centromeres during cell division. The data suggest that in plants NDC80 is on par with 'foundation' kinetochore proteins such as CENH3 and CENP-C.