Literature DB >> 17643108

High-throughput cloning and expression in recalcitrant bacteria.

Eric R Geertsma1, Bert Poolman.   

Abstract

We developed a generic method for high-throughput cloning in bacteria that are less amenable to conventional DNA manipulations. The method involves ligation-independent cloning in an intermediary Escherichia coli vector, which is rapidly converted via vector-backbone exchange (VBEx) into an organism-specific plasmid ready for high-efficiency transformation. We demonstrated VBEx proof of principle for Lactococcus lactis, but the method can be adapted to all organisms for which plasmids are available.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17643108     DOI： 10.1038/nmeth1073

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  46 in total

1.  Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation.

Authors:  Michael Hohl; Christophe Briand; Markus G Grütter; Markus A Seeger
Journal:  Nat Struct Mol Biol       Date:  2012-03-25       Impact factor: 15.369

2.  Quaternary structure and functional unit of energy coupling factor (ECF)-type transporters.

Authors:  Josy ter Beek; Ria H Duurkens; Guus B Erkens; Dirk Jan Slotboom
Journal:  J Biol Chem       Date:  2010-12-06       Impact factor: 5.157

3.  Processing and maturation of the pilin of the type IV secretion system encoded within the gonococcal genetic island.

Authors:  Samta Jain; Jörg Kahnt; Chris van der Does
Journal:  J Biol Chem       Date:  2011-10-17       Impact factor: 5.157

4.  The rate of folding dictates substrate secretion by the Escherichia coli hemolysin type 1 secretion system.

Authors:  Patrick J Bakkes; Stefan Jenewein; Sander H J Smits; I Barry Holland; Lutz Schmitt
Journal:  J Biol Chem       Date:  2010-10-22       Impact factor: 5.157

5.  The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter.

Authors:  Christopher Mulligan; Eric R Geertsma; Emmanuele Severi; David J Kelly; Bert Poolman; Gavin H Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  2009-01-28       Impact factor: 11.205

6.  Quality control of overexpressed membrane proteins.

Authors:  Eric R Geertsma; Maarten Groeneveld; Dirk-Jan Slotboom; Bert Poolman
Journal:  Proc Natl Acad Sci U S A       Date:  2008-04-07       Impact factor: 11.205

7.  Conformational dynamics in substrate-binding domains influences transport in the ABC importer GlnPQ.

Authors:  Giorgos Gouridis; Gea K Schuurman-Wolters; Evelyn Ploetz; Florence Husada; Ruslan Vietrov; Marijn de Boer; Thorben Cordes; Bert Poolman
Journal:  Nat Struct Mol Biol       Date:  2014-12-08       Impact factor: 15.369

8.  Regulation of acetate kinase isozymes and its importance for mixed-acid fermentation in Lactococcus lactis.

Authors:  Pranav Puri; Anisha Goel; Agnieszka Bochynska; Bert Poolman
Journal:  J Bacteriol       Date:  2014-01-24       Impact factor: 3.490

9.  Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis.

Authors:  Ravi K R Marreddy; Eric R Geertsma; Hjalmar P Permentier; Joao P C Pinto; Jan Kok; Bert Poolman
Journal:  PLoS One       Date:  2010-04-26       Impact factor: 3.240

10.  Lactococcus lactis, an alternative system for functional expression of peripheral and intrinsic Arabidopsis membrane proteins.

Authors:  Annie Frelet-Barrand; Sylvain Boutigny; Lucas Moyet; Aurélien Deniaud; Daphné Seigneurin-Berny; Daniel Salvi; Florent Bernaudat; Pierre Richaud; Eva Pebay-Peyroula; Jacques Joyard; Norbert Rolland
Journal:  PLoS One       Date:  2010-01-20       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.