BACKGROUND: Group A rotavirus has been recognized as the major etiologic agent of childhood gastroenteritis in infants and young children worldwide. Rapid progress towards the development of an efficacious rotavirus vaccine has warranted extensive epidemiological studies on rotavirus serotypes that cause severe disease in developing and developed countries and to monitor the emergence of newer and unusual strains in different geographical settings that could represent variants not covered by existing vaccines. METHODS: In this study, we determined the prevalence of rotavirus infection and characterised group A rotavirus in stool samples by using monoclonal antibody (MAb) based ELISA and polyacrylamide gel electrophoresis (PAGE). Stool samples were collected from 745 children of 0-3 years of age presenting to the hospital with acute diarrhea between March 1995 and August 1999. These were assayed for antigenic (group, subgroup, serotype) and genomic (viral RNA profile and VP7 and VP4 genotype) characterization by ELISA and PAGE. RESULTS: Out of 745 stool samples analysed 168 were found to be positive for rotavirus. Among these 118 could be assigned a subgroup (SG), serotype and electropherotype (E-type). The study has evidenced the predominant occurrence of strains with short E-type, SGI and serotype G2 in 66.1% of the samples. The presence of strains representing 10 different E-types and mixed genotype specificities with G2 P[4,8] and G1-G2 P[4,8] has documented the prevailing high genomic diversity of rotaviruses in this geographical area. CONCLUSION: This study has described the predominant strains of rotavirus in south India. There is a need for further detailed studies on the molecular characterization of rotaviruses which would have important implications in vaccine evaluation programmes.
BACKGROUND:Group A rotavirus has been recognized as the major etiologic agent of childhood gastroenteritis in infants and young children worldwide. Rapid progress towards the development of an efficacious rotavirus vaccine has warranted extensive epidemiological studies on rotavirus serotypes that cause severe disease in developing and developed countries and to monitor the emergence of newer and unusual strains in different geographical settings that could represent variants not covered by existing vaccines. METHODS: In this study, we determined the prevalence of rotavirus infection and characterised group A rotavirus in stool samples by using monoclonal antibody (MAb) based ELISA and polyacrylamide gel electrophoresis (PAGE). Stool samples were collected from 745 children of 0-3 years of age presenting to the hospital with acute diarrhea between March 1995 and August 1999. These were assayed for antigenic (group, subgroup, serotype) and genomic (viral RNA profile and VP7 and VP4 genotype) characterization by ELISA and PAGE. RESULTS: Out of 745 stool samples analysed 168 were found to be positive for rotavirus. Among these 118 could be assigned a subgroup (SG), serotype and electropherotype (E-type). The study has evidenced the predominant occurrence of strains with short E-type, SGI and serotype G2 in 66.1% of the samples. The presence of strains representing 10 different E-types and mixed genotype specificities with G2 P[4,8] and G1-G2 P[4,8] has documented the prevailing high genomic diversity of rotaviruses in this geographical area. CONCLUSION: This study has described the predominant strains of rotavirus in south India. There is a need for further detailed studies on the molecular characterization of rotaviruses which would have important implications in vaccine evaluation programmes.