Literature DB >> 17635661

A sandwich-embedding method for oriented sectioning.

O Leroux1, G Van der Kinderen, R L L Viane.   

Abstract

High-quality sections are indispensable for many scientific studies. Most published methods are often time-consuming or require special devices. We present an easy, quick and low-cost method for oriented embedding of thin structures using glycol methacrylate resin and self-constructed, reusable embedding tools made of overhead transparencies. This technique allows for more flexibility in orientation than other methods, enabling precise transverse, longitudinal and even oblique sectioning.

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Year:  2007        PMID: 17635661     DOI: 10.1111/j.1365-2818.2007.01786.x

Source DB:  PubMed          Journal:  J Microsc        ISSN: 0022-2720            Impact factor:   1.758


  8 in total

1.  Non-lignified helical cell wall thickenings in root cortical cells of Aspleniaceae (Polypodiales): histology and taxonomical significance.

Authors:  O Leroux; A Bagniewska-Zadworna; S K Rambe; J P Knox; S E Marcus; E Bellefroid; D Stubbe; B Chabbert; A Habrant; M Claeys; R L L Viane
Journal:  Ann Bot       Date:  2010-11-29       Impact factor: 4.357

2.  Peripheral axons of the adult zebrafish maxillary barbel extensively remyelinate during sensory appendage regeneration.

Authors:  Alex C Moore; Tiffany E Mark; Ann K Hogan; Jacek Topczewski; Elizabeth E LeClair
Journal:  J Comp Neurol       Date:  2012-12-15       Impact factor: 3.215

3.  An extensin-rich matrix lines the carinal canals in Equisetum ramosissimum, which may function as water-conducting channels.

Authors:  O Leroux; J P Knox; B Masschaele; A Bagniewska-Zadworna; S E Marcus; M Claeys; L van Hoorebeke; R L L Viane
Journal:  Ann Bot       Date:  2011-07-12       Impact factor: 4.357

4.  Mechanical fixation techniques for processing and orienting delicate samples, such as the root of Arabidopsis thaliana, for light or electron microscopy.

Authors:  Shuang Wu; Tobias I Baskin; Kimberly L Gallagher
Journal:  Nat Protoc       Date:  2012-05-17       Impact factor: 13.491

5.  Heterogeneity of silica and glycan-epitope distribution in epidermal idioblast cell walls in Adiantum raddianum laminae.

Authors:  Olivier Leroux; Frederic Leroux; Alexandra Antunes Mastroberti; Fernanda Santos-Silva; Denis Van Loo; Agnieszka Bagniewska-Zadworna; Luc Van Hoorebeke; Sara Bals; Zoë A Popper; Jorge Ernesto de Araujo Mariath
Journal:  Planta       Date:  2013-02-22       Impact factor: 4.116

6.  ALTERED MERISTEM PROGRAM1 suppresses ectopic stem cell niche formation in the shoot apical meristem in a largely cytokinin-independent manner.

Authors:  Wenwen Huang; Delphine Pitorre; Olena Poretska; Christine Marizzi; Nikola Winter; Brigitte Poppenberger; Tobias Sieberer
Journal:  Plant Physiol       Date:  2015-02-11       Impact factor: 8.340

7.  Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.

Authors:  Olivier Leroux; Michaela Eder; Friederike Saxe; John W C Dunlop; Zoë A Popper; Ronald L L Viane; J Paul Knox
Journal:  Ann Bot       Date:  2018-02-12       Impact factor: 4.357

8.  The Last Half Century of Fish Explant and Organ Culture.

Authors:  Elizabeth E LeClair
Journal:  Zebrafish       Date:  2021-01-18       Impact factor: 1.985

  8 in total

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