Literature DB >> 17635143

Monitoring interactions between G-protein-coupled receptors and beta-arrestins.

K D G Pfleger1, M B Dalrymple, J R Dromey, K A Eidne.   

Abstract

beta-Arrestins 1 and 2 are ubiquitously expressed intracellular adaptor and scaffolding proteins that play important roles in GPCR (G-protein-coupled receptor) desensitization, internalization, intracellular trafficking and G-protein-independent signalling. Recent developments in BRET (bioluminescence resonance energy transfer) technology enable novel insights to be gained from real-time monitoring of GPCR-beta-arrestin complexes in live cells for prolonged periods. In concert with confocal microscopy, assays for studying internalization and recycling kinetics such as ELISAs, and techniques for measuring downstream signalling pathways such as those involving MAPKs (mitogen-activated protein kinases), investigators can now use a range of experimental tools to elucidate the ever-expanding roles of beta-arrestins in mediating GPCR function.

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Year:  2007        PMID： 17635143     DOI： 10.1042/BST0350764

Source DB:  PubMed          Journal:  Biochem Soc Trans        ISSN： 0300-5127            Impact factor:   5.407


  8 in total

Review 1.  Allostery at G protein-coupled receptor homo- and heteromers: uncharted pharmacological landscapes.

Authors:  Nicola J Smith; Graeme Milligan
Journal:  Pharmacol Rev       Date:  2010-12       Impact factor: 25.468

2.  Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Gα₁₃ and β-arrestin-2.

Authors:  Laura Jenkins; Elisa Alvarez-Curto; Kate Campbell; Sabrina de Munnik; Meritxell Canals; Sabine Schlyer; Graeme Milligan
Journal:  Br J Pharmacol       Date:  2011-02       Impact factor: 8.739

3.  Temporal profiling of orexin receptor-arrestin-ubiquitin complexes reveals differences between receptor subtypes.

Authors:  Matthew B Dalrymple; Werner C Jaeger; Karin A Eidne; Kevin D G Pfleger
Journal:  J Biol Chem       Date:  2011-03-04       Impact factor: 5.157

4.  Agonist-independent interactions between beta-arrestins and mutant vasopressin type II receptors associated with nephrogenic syndrome of inappropriate antidiuresis.

Authors:  Martina Kocan; Heng B See; Natália G Sampaio; Karin A Eidne; Brian J Feldman; Kevin D G Pfleger
Journal:  Mol Endocrinol       Date:  2009-01-29

5.  Site-specific phosphorylation of CXCR4 is dynamically regulated by multiple kinases and results in differential modulation of CXCR4 signaling.

Authors:  John M Busillo; Sylvain Armando; Rajarshi Sengupta; Olimpia Meucci; Michel Bouvier; Jeffrey L Benovic
Journal:  J Biol Chem       Date:  2010-01-04       Impact factor: 5.157

6.  Setting Up a Bioluminescence Resonance Energy Transfer High throughput Screening Assay to Search for Protein/Protein Interaction Inhibitors in Mammalian Cells.

Authors:  Cyril Couturier; Benoit Deprez
Journal:  Front Endocrinol (Lausanne)       Date:  2012-09-11       Impact factor: 5.555

7.  Enhanced BRET Technology for the Monitoring of Agonist-Induced and Agonist-Independent Interactions between GPCRs and β-Arrestins.

Authors:  Martina Kocan; Matthew B Dalrymple; Ruth M Seeber; Brian J Feldman; Kevin D G Pfleger
Journal:  Front Endocrinol (Lausanne)       Date:  2011-01-14       Impact factor: 5.555

8.  BRET biosensors to study GPCR biology, pharmacology, and signal transduction.

Authors:  Ali Salahpour; Stefano Espinoza; Bernard Masri; Vincent Lam; Larry S Barak; Raul R Gainetdinov
Journal:  Front Endocrinol (Lausanne)       Date:  2012-08-29       Impact factor: 5.555
  8 in total

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