OBJECTIVE: To investigate the effects of mangiferin on telomerase activity and apoptosis in K562 cells lines and study the melocular mechanism of it's antileukemic. METHODS: Cell apoptosis was observed by light microscopy and transmission electron microscopy; The expression of Fas was measured by flow cytometry; Polymerase chain reaction enzyme linked immunoassay (PCR-ELISA) was used to assay telomerase activity of K562 cells. RESULTS: Mangiferin could inhibit telomerase activity of K562 cells in a time-and-concentration-dependent manner. Meanwhile, it could induce apoptosis obviously and up-regulate the levels of Fas in K562 cells. CONCLUSION: Mangiferin can inhibit telomerase activity of K562 cells, and the mechanism of effect is maybe relate to inducing apoptosis and the expression of Fas protein.
OBJECTIVE: To investigate the effects of mangiferin on telomerase activity and apoptosis in K562 cells lines and study the melocular mechanism of it's antileukemic. METHODS: Cell apoptosis was observed by light microscopy and transmission electron microscopy; The expression of Fas was measured by flow cytometry; Polymerase chain reaction enzyme linked immunoassay (PCR-ELISA) was used to assay telomerase activity of K562 cells. RESULTS: Mangiferin could inhibit telomerase activity of K562 cells in a time-and-concentration-dependent manner. Meanwhile, it could induce apoptosis obviously and up-regulate the levels of Fas in K562 cells. CONCLUSION: Mangiferin can inhibit telomerase activity of K562 cells, and the mechanism of effect is maybe relate to inducing apoptosis and the expression of Fas protein.
Authors: Muhammad Imran; Muhammad Sajid Arshad; Masood Sadiq Butt; Joong-Ho Kwon; Muhammad Umair Arshad; Muhammad Tauseef Sultan Journal: Lipids Health Dis Date: 2017-05-02 Impact factor: 3.876