A Asanbaeva1, K Masuda, E J-M A Thonar, S M Klisch, R L Sah. 1. Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla, CA 92093-0412, USA.
Abstract
OBJECTIVE: To examine the effect of beta-aminopropionitrile (BAPN), an inhibitor of lysyl oxidase, on growth and remodeling of immature articular cartilage in vitro. DESIGN: Immature bovine articular cartilage explants from the superficial and middle layers were cultured for 13 days in serum-containing medium with or without BAPN. Variations in tissue size, accumulation of proteoglycan and collagen (COL), and tensile mechanical properties were assessed. RESULTS: The inclusion of serum resulted in expansive tissue growth, stimulation of proteoglycan and COL deposition, and a diminution of tensile integrity. Supplementation of medium with BAPN accentuated this phenotype in terms of a further increase in tissue size in explants from the superficial layer and further diminution of tensile integrity, without affecting the contents of proteoglycan and COL in explants from both the superficial and middle layers. CONCLUSION: COL crosslinking is a major factor in modulating the phenotype of cartilage growth and the associated balance between proteoglycan content and integrity of the COL network.
OBJECTIVE: To examine the effect of beta-aminopropionitrile (BAPN), an inhibitor of lysyl oxidase, on growth and remodeling of immature articular cartilage in vitro. DESIGN: Immature bovinearticular cartilage explants from the superficial and middle layers were cultured for 13 days in serum-containing medium with or without BAPN. Variations in tissue size, accumulation of proteoglycan and collagen (COL), and tensile mechanical properties were assessed. RESULTS: The inclusion of serum resulted in expansive tissue growth, stimulation of proteoglycan and COL deposition, and a diminution of tensile integrity. Supplementation of medium with BAPN accentuated this phenotype in terms of a further increase in tissue size in explants from the superficial layer and further diminution of tensile integrity, without affecting the contents of proteoglycan and COL in explants from both the superficial and middle layers. CONCLUSION: COL crosslinking is a major factor in modulating the phenotype of cartilage growth and the associated balance between proteoglycan content and integrity of the COL network.
Authors: Woojin M Han; Nandan L Nerurkar; Lachlan J Smith; Nathan T Jacobs; Robert L Mauck; Dawn M Elliott Journal: Ann Biomed Eng Date: 2012-07 Impact factor: 3.934
Authors: Stephen M Klisch; Anna Asanbaeva; Sevan R Oungoulian; Koichi Masuda; Eugene J-Ma Thonar; Andrew Davol; Robert L Sah Journal: J Biomech Eng Date: 2008-06 Impact factor: 2.097
Authors: Woojin M Han; Su-Jin Heo; Tristan P Driscoll; Lachlan J Smith; Robert L Mauck; Dawn M Elliott Journal: Biophys J Date: 2013-08-06 Impact factor: 4.033
Authors: Robert J Nims; Alexander D Cigan; Krista M Durney; Brian K Jones; John D O'Neill; Wing-Sum A Law; Gordana Vunjak-Novakovic; Clark T Hung; Gerard A Ateshian Journal: Tissue Eng Part A Date: 2017-03-27 Impact factor: 3.845
Authors: Eleftherios A Makris; Regina F MacBarb; Donald J Responte; Jerry C Hu; Kyriacos A Athanasiou Journal: FASEB J Date: 2013-03-01 Impact factor: 5.191
Authors: Thomas J Kean; Hisashi Mera; G Adam Whitney; Danielle L MacKay; Amad Awadallah; Russell J Fernandes; James E Dennis Journal: Connect Tissue Res Date: 2016-04-29 Impact factor: 3.417