OBJECTIVE: We present the largest longitudinal study to date that examines the association between Kaposi's Sarcoma (KS) disease progression and the presence and viral load of human herpesvirus 8 (HHV-8). METHODS: Ninety-six men were enrolled at HIV clinics in Atlanta, Georgia, who had KS (n = 47) or were without KS but seropositive for HHV-8. Visits occurred at 6-month intervals for 2 years at which the patient's KS status was evaluated and oral fluid and blood were collected for quantification of HHV-8 DNA and antibodies. RESULTS: The presence of HHV-8 DNA in blood was more common (P < 0.001) and the viral load higher (P < 0.001) in men with KS in comparison with men without KS. Mean HHV-8 viral loads in blood and oral fluids were associated with disease status, being highest among patients with progressing KS, intermediate among patients with stable KS, and lowest among patients with regressing KS. Consistent with our previous report high antibody titers to HHV-8 orf 65 were inversely associated with HHV-8 shedding in oral fluid. CONCLUSIONS: We observed a significant association between changes in KS disease severity and the presence and viral load of HHV-8. HHV-8 viral load in blood may provide useful information to clinicians for assessment of the risk of further disease progression in patients with KS.
OBJECTIVE: We present the largest longitudinal study to date that examines the association between Kaposi's Sarcoma (KS) disease progression and the presence and viral load of human herpesvirus 8 (HHV-8). METHODS: Ninety-six men were enrolled at HIV clinics in Atlanta, Georgia, who had KS (n = 47) or were without KS but seropositive for HHV-8. Visits occurred at 6-month intervals for 2 years at which the patient's KS status was evaluated and oral fluid and blood were collected for quantification of HHV-8 DNA and antibodies. RESULTS: The presence of HHV-8 DNA in blood was more common (P < 0.001) and the viral load higher (P < 0.001) in men with KS in comparison with men without KS. Mean HHV-8 viral loads in blood and oral fluids were associated with disease status, being highest among patients with progressing KS, intermediate among patients with stable KS, and lowest among patients with regressing KS. Consistent with our previous report high antibody titers to HHV-8 orf 65 were inversely associated with HHV-8 shedding in oral fluid. CONCLUSIONS: We observed a significant association between changes in KS disease severity and the presence and viral load of HHV-8. HHV-8 viral load in blood may provide useful information to clinicians for assessment of the risk of further disease progression in patients with KS.
Authors: Gundolf Schüttfort; Timo Wolf; Phillip de Leuw; Gerrit Kann; Maximilian Blume; Hans-Reinhard Brodt; Christoph Stephan Journal: Infection Date: 2015-05-07 Impact factor: 3.553
Authors: George K Siberry; Mark J Abzug; Sharon Nachman; Michael T Brady; Kenneth L Dominguez; Edward Handelsman; Lynne M Mofenson; Steve Nesheim Journal: Pediatr Infect Dis J Date: 2013-11 Impact factor: 2.129
Authors: Martin M Nsubuga; Robert J Biggar; Susan Combs; Vickie Marshall; Georgina Mbisa; Fred Kambugu; Meghna Mehta; Benon Biryahwaho; Charles S Rabkin; Denise Whitby; Sam M Mbulaiteye Journal: Cancer Lett Date: 2008-01-30 Impact factor: 8.679
Authors: Kristen M Tamburro; Dongmei Yang; Jessica Poisson; Yuri Fedoriw; Debasmita Roy; Amy Lucas; Sang-Hoon Sin; Nadia Malouf; Vincent Moylan; Blossom Damania; Stephan Moll; Charles van der Horst; Dirk P Dittmer Journal: Virology Date: 2012-08-24 Impact factor: 3.616
Authors: Christine Johnston; Jackson Orem; Fred Okuku; Mary Kalinaki; Misty Saracino; Edward Katongole-Mbidde; Merle Sande; Allan Ronald; Keith McAdam; Meei-Li Huang; Linda Drolette; Stacy Selke; Anna Wald; Lawrence Corey; Corey Casper Journal: PLoS One Date: 2009-01-20 Impact factor: 3.240