OBJECTIVES: This study was undertaken to investigate if there is a disparity in the antioxidant status and the ability of superoxide anion (O(2)(-)) generation in the patients with acute myeloid leukemia (AML). DESIGN AND METHODS: The peripheral blood samples from thirty AML patients and thirty-six healthy subjects were collected and leukocytes, erythrocytes and plasma were separated for use in various parameter measurements. RESULTS: The generation of O(2)(-), as reflected by lucigenin-based CL (LBCL), by the leukocytes of patients with AML was found to be significantly elevated either in resting or stimuli-elicited condition as compared with that of healthy controls (p<0.05). Coincidentally, these data were matched up with the suppressed SOD activities, notably in Cu/Zn SOD isoform found in AML patients (p<0.05). Conversely, SOD and GPx activities in erythrocytes of patients with AML were shown to be significantly higher than their normal counterparts (p<0.05). CONCLUSIONS: These data suggest that altered expression of antioxidant enzymes and higher capability of O(2)(-) generation by leukocytes seem to be a distinct feature of AML.
OBJECTIVES: This study was undertaken to investigate if there is a disparity in the antioxidant status and the ability of superoxide anion (O(2)(-)) generation in the patients with acute myeloid leukemia (AML). DESIGN AND METHODS: The peripheral blood samples from thirty AMLpatients and thirty-six healthy subjects were collected and leukocytes, erythrocytes and plasma were separated for use in various parameter measurements. RESULTS: The generation of O(2)(-), as reflected by lucigenin-based CL (LBCL), by the leukocytes of patients with AML was found to be significantly elevated either in resting or stimuli-elicited condition as compared with that of healthy controls (p<0.05). Coincidentally, these data were matched up with the suppressed SOD activities, notably in Cu/Zn SOD isoform found in AMLpatients (p<0.05). Conversely, SOD and GPx activities in erythrocytes of patients with AML were shown to be significantly higher than their normal counterparts (p<0.05). CONCLUSIONS: These data suggest that altered expression of antioxidant enzymes and higher capability of O(2)(-) generation by leukocytes seem to be a distinct feature of AML.
Authors: Shanshan Pei; Mohammad Minhajuddin; Angelo D'Alessandro; Travis Nemkov; Brett M Stevens; Biniam Adane; Nabilah Khan; Fred K Hagen; Vinod K Yadav; Subhajyoti De; John M Ashton; Kirk C Hansen; Jonathan A Gutman; Daniel A Pollyea; Peter A Crooks; Clayton Smith; Craig T Jordan Journal: J Biol Chem Date: 2016-08-29 Impact factor: 5.157
Authors: Shanshan Pei; Mohammad Minhajuddin; Kevin P Callahan; Marlene Balys; John M Ashton; Sarah J Neering; Eleni D Lagadinou; Cheryl Corbett; Haobin Ye; Jane L Liesveld; Kristen M O'Dwyer; Zheng Li; Lei Shi; Patricia Greninger; Jeffrey Settleman; Cyril Benes; Fred K Hagen; Joshua Munger; Peter A Crooks; Michael W Becker; Craig T Jordan Journal: J Biol Chem Date: 2013-10-02 Impact factor: 5.157
Authors: Chiou-Hong Lin; John P Vu; Chen-Yen Yang; Mint Sirisawad; Chun-Te Chen; Hung Dao; Jing Liu; Xuan Ma; Chin Pan; Joseph Cefalu; Chris Tse; Erica Jackson; Hsu-Ping Kuo Journal: Am J Cancer Res Date: 2021-06-15 Impact factor: 6.166