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Literature DB >> 17628150

Targeting two-component signal transduction: a novel drug discovery system.

Ario Okada¹, Yasuhiro Gotoh, Takafumi Watanabe, Eiji Furuta, Kaneyoshi Yamamoto, Ryutaro Utsumi.

Abstract

We have developed two screening systems for isolating inhibitors that target bacterial two-component signal transduction: (1) a differential growth assay using a temperature-sensitive yycF mutant (CNM2000) of Bacillus subtilis, which is supersensitive to histidine kinase inhibitors, and (2) a high-throughput genetic system for targeting the homodimerization of histidine kinases essential for the bacterial two-component signal transduction. By using these methods, we have been able to identify various types of inhibitors that block the autophosphorylation of histidine kinases with different modes of actions.

Entities: Species

Mesh：

Substances：
Bacterial Proteins

Year: 2007 PMID： 17628150 DOI： 10.1016/S0076-6879(06)22019-6

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

19 in total

1. Development and validation of a high-throughput cell-based screen to identify activators of a bacterial two-component signal transduction system.

Authors: Julia J van Rensburg; Kate R Fortney; Lan Chen; Andrew J Krieger; Bruno P Lima; Alan J Wolfe; Barry P Katz; Zhong-Yin Zhang; Stanley M Spinola
Journal: Antimicrob Agents Chemother Date: 2015-04-13 Impact factor: 5.191

2. Development of an antivirulence drug against Streptococcus mutans: repression of biofilm formation, acid tolerance, and competence by a histidine kinase inhibitor, walkmycin C.

Authors: Yoko Eguchi; Norihiro Kubo; Hiroko Matsunaga; Masayuki Igarashi; Ryutaro Utsumi
Journal: Antimicrob Agents Chemother Date: 2011-01-31 Impact factor: 5.191

3. Essentiality, bypass, and targeting of the YycFG (VicRK) two-component regulatory system in gram-positive bacteria.

Authors: Malcolm E Winkler; James A Hoch
Journal: J Bacteriol Date: 2008-02-01 Impact factor: 3.490

4. The two-component system QseEF and the membrane protein QseG link adrenergic and stress sensing to bacterial pathogenesis.

Authors: Nicola C Reading; David A Rasko; Alfredo G Torres; Vanessa Sperandio
Journal: Proc Natl Acad Sci U S A Date: 2009-03-16 Impact factor: 11.205

Targeting two-component signal transduction: a novel drug discovery system.

1. Development and validation of a high-throughput cell-based screen to identify activators of a bacterial two-component signal transduction system.

2. Development of an antivirulence drug against Streptococcus mutans: repression of biofilm formation, acid tolerance, and competence by a histidine kinase inhibitor, walkmycin C.

3. Essentiality, bypass, and targeting of the YycFG (VicRK) two-component regulatory system in gram-positive bacteria.

4. The two-component system QseEF and the membrane protein QseG link adrenergic and stress sensing to bacterial pathogenesis.

5. Role of the PAS sensor domains in the Bacillus subtilis sporulation kinase KinA.

Review 6. Bacterial biofilms: development, dispersal, and therapeutic strategies in the dawn of the postantibiotic era.

7. WalK, the Path towards New Antibacterials with Low Potential for Resistance Development.

Review 8. Protein histidine kinases: assembly of active sites and their regulation in signaling pathways.

Review 9. Synthesis of histidine kinase inhibitors and their biological properties.

10. Mechanistic insight into inhibition of two-component system signaling.