| Literature DB >> 17623938 |
Yan-Dong Wang1, Randolph B Caldwell.
Abstract
Nuclear and cytoplasmic proteins from DT40 B cells are extracted by serial fractionation in this protocol. The protein extracts are suitable for the detection of DNA-protein interaction, protein-protein interaction, DNase I footprinting analysis, and related techniques. This is a general protocol that was adapted as shown here to our use due to the fragile nature of DT40 cells and their nuclei.Mesh：
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Year: 2006 PMID： 17623938 DOI： 10.1007/978-1-4020-4896-8_43
Source DB: PubMed Journal: Subcell Biochem ISSN： 0306-0225