OBJECTIVE: L-thyroxine (T4), the most widely used drug for hypothyroidism, undergoes glucuronidation by UDP-glucuronosyltransferases. Clinical evidence obtained after the administration of anticonvulsants suggest that glucuronidation may play an important role in T4 homeostasis in humans. The aims of this study were to determine the T4 glucuronidation ability of all commercially available human UGTs, and investigate the relationship between genetic polymorphisms in UGT1A1 and UGT1A9 and T4 glucuronidation in human livers. METHODS: Glucuronidation of T4 in human liver microsomes and recombinant UDP-glucuronosyltransferases was measured by high-pressure liquid chromatography. UGT1A1 -53(TA)6>7 (UGT1A1*28) and UGT1A9 -118T9>10 (UGT1A9*1b) variants were genotyped by polymerase chain reaction and sizing. RESULTS: A strong correlation was observed between the glucuronidation of T4 and SN-38, a UGT1A1 substrate (r=0.82, P<0.0001). A significant trend of decreasing T4 glucuronide (T4G) levels was observed with increasing number of UGT1A1 -53(TA)7 alleles (P=0.001). Other hepatic UDP-glucuronosyltransferases involved in T4G formation are UGT1A3 and UGT1A9. No significant relationship was observed between UGT1A9 -118T9>10 and T4 glucuronidation activity. T4 can also undergo glucuronidation by UGT1A8 and UGT1A10, which are expressed in the gastrointestinal tract (but not the liver) and may be important for first-pass T4 metabolism.
OBJECTIVE:L-thyroxine (T4), the most widely used drug for hypothyroidism, undergoes glucuronidation by UDP-glucuronosyltransferases. Clinical evidence obtained after the administration of anticonvulsants suggest that glucuronidation may play an important role in T4 homeostasis in humans. The aims of this study were to determine the T4 glucuronidation ability of all commercially available human UGTs, and investigate the relationship between genetic polymorphisms in UGT1A1 and UGT1A9 and T4 glucuronidation in human livers. METHODS: Glucuronidation of T4 in human liver microsomes and recombinant UDP-glucuronosyltransferases was measured by high-pressure liquid chromatography. UGT1A1 -53(TA)6>7 (UGT1A1*28) and UGT1A9 -118T9>10 (UGT1A9*1b) variants were genotyped by polymerase chain reaction and sizing. RESULTS: A strong correlation was observed between the glucuronidation of T4 and SN-38, a UGT1A1 substrate (r=0.82, P<0.0001). A significant trend of decreasing T4 glucuronide (T4G) levels was observed with increasing number of UGT1A1 -53(TA)7 alleles (P=0.001). Other hepatic UDP-glucuronosyltransferases involved in T4G formation are UGT1A3 and UGT1A9. No significant relationship was observed between UGT1A9 -118T9>10 and T4 glucuronidation activity. T4 can also undergo glucuronidation by UGT1A8 and UGT1A10, which are expressed in the gastrointestinal tract (but not the liver) and may be important for first-pass T4 metabolism.
Authors: Landry K Kamdem; Yong Liu; Vered Stearns; Susan A Kadlubar; Jacqueline Ramirez; Stacie Jeter; Karineh Shahverdi; Bryan A Ward; Evan Ogburn; Mark J Ratain; David A Flockhart; Zeruesenay Desta Journal: Br J Clin Pharmacol Date: 2010-12 Impact factor: 4.335
Authors: Federico Innocenti; Jacqueline Ramírez; Jennifer Obel; Julia Xiong; Snezana Mirkov; Yi-Lin Chiu; David A Katz; Robert A Carr; Wei Zhang; Soma Das; Araba Adjei; Ann M Moyer; Pei Xian Chen; Andrew Krivoshik; Diane Medina; Gary B Gordon; Mark J Ratain; Leonardo Sahelijo; Richard M Weinshilboum; Gini F Fleming; Anahita Bhathena Journal: Pharmacogenet Genomics Date: 2013-07 Impact factor: 2.089
Authors: Ana B Santoro; Daniela D Vargens; Mateus de Camargo Barros Filho; Daniel A Bulzico; Luiz Paulo Kowalski; Ricardo M R Meirelles; Daniela P Paula; Ronaldo R S Neves; Cencita N Pessoa; Claudio J Struchine; Guilherme Suarez-Kurtz Journal: Br J Clin Pharmacol Date: 2014-11 Impact factor: 4.335