Literature DB >> 17620374

Detection of azithromycin resistance in Treponema pallidum by real-time PCR.

Mark W Pandori1, Charmie Gordones, Lina Castro, Joseph Engelman, Mark Siedner, Sheila Lukehart, Jeffrey Klausner.   

Abstract

We describe a real-time PCR assay for the discrimination of azithromycin-resistant and -susceptible strains of Treponema pallidum. This assay is rapid and allows for as many as 30 clinical specimens to be analyzed simultaneously without the need for DNA sequencing.

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Year:  2007        PMID： 17620374      PMCID： PMC2043243          DOI： 10.1128/AAC.00340-07

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  23 in total

1.  A point mutation associated with bacterial macrolide resistance is present in both 23S rRNA genes of an erythromycin-resistant Treponema pallidum clinical isolate.

Authors:  L V Stamm; H L Bergen
Journal:  Antimicrob Agents Chemother       Date:  2000-03       Impact factor: 5.191

2.  Use of directly observed azithromycin treatment for syphilis in a homeless woman.

Authors:  Doug Campos-Outcalt; Steve Hurwitz; Tom Mickey
Journal:  Sex Transm Dis       Date:  2002-06       Impact factor: 2.830

3.  Azithromycin-resistant syphilis infection: San Francisco, California, 2000-2004.

Authors:  Samuel J Mitchell; Joseph Engelman; Charlotte K Kent; Sheila A Lukehart; Charmie Godornes; Jeffrey D Klausner
Journal:  Clin Infect Dis       Date:  2005-12-28       Impact factor: 9.079

4.  Diagnosis of Treponema pallidum in vitreous samples using real time polymerase chain reaction.

Authors:  M S Rajan; P Pantelidis; C Y W Tong; G L French; E M Graham; M R Stanford
Journal:  Br J Ophthalmol       Date:  2006-05       Impact factor: 4.638

5.  Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum.

Authors:  A G Koek; S M Bruisten; M Dierdorp; A P van Dam; K Templeton
Journal:  Clin Microbiol Infect       Date:  2006-12       Impact factor: 8.067

6.  Diagnosis of gastric syphilis by direct immunofluorescence staining and real-time PCR testing.

Authors:  Cheng-Yen Chen; Kai-Hua Chi; Robert W George; David L Cox; Amitabh Srivastava; Mário Rui Silva; Fátima Carneiro; Gregory Y Lauwers; Ronald C Ballard
Journal:  J Clin Microbiol       Date:  2006-09       Impact factor: 5.948

7.  New tests for syphilis: rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene.

Authors:  H Liu; B Rodes; C Y Chen; B Steiner
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

8.  Development of a real-time PCR assay to detect Treponema pallidum in clinical specimens and assessment of the assay's performance by comparison with serological testing.

Authors:  David E Leslie; Franca Azzato; Theo Karapanagiotidis; Jennie Leydon; Janet Fyfe
Journal:  J Clin Microbiol       Date:  2006-10-25       Impact factor: 5.948

9.  Detection of Treponema pallidum in the vitreous by PCR.

Authors:  M Müller; I Ewert; F Hansmann; C Tiemann; H J Hagedorn; W Solbach; J Roider; B Nölle; H Laqua; H Hoerauf
Journal:  Br J Ophthalmol       Date:  2006-11-15       Impact factor: 4.638

10.  A randomized, comparative pilot study of azithromycin versus benzathine penicillin G for treatment of early syphilis.

Authors:  Edward W Hook; David H Martin; Joan Stephens; Babara S Smith; Kim Smith
Journal:  Sex Transm Dis       Date:  2002-08       Impact factor: 2.830
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  6 in total

Review 1.  The endemic treponematoses.

Authors:  Lorenzo Giacani; Sheila A Lukehart
Journal:  Clin Microbiol Rev       Date:  2014-01       Impact factor: 26.132

2.  Gene subtype analysis of Treponema pallidum for drug resistance to azithromycin.

Authors:  Yuecui Li; Jin Li; Weiyue Hu; Hongxia Luo; Jing Zhou; Chenghang Li; Chunjiao Chen
Journal:  Exp Ther Med       Date:  2018-05-30       Impact factor: 2.447

3.  Detection of the A2058G and A2059G 23S rRNA gene point mutations associated with azithromycin resistance in Treponema pallidum by use of a TaqMan real-time multiplex PCR assay.

Authors:  Cheng-Yen Chen; Kai-Hua Chi; Allan Pillay; Eli Nachamkin; John R Su; Ronald C Ballard
Journal:  J Clin Microbiol       Date:  2013-01-02       Impact factor: 5.948

Review 4.  Global challenge of antibiotic-resistant Treponema pallidum.

Authors:  Lola V Stamm
Journal:  Antimicrob Agents Chemother       Date:  2009-10-05       Impact factor: 5.191

5.  Characterizing the syphilis epidemic among men who have sex with men in Lima, Peru to identify new treatment and control strategies.

Authors:  Robert G Deiss; Segundo R Leon; Kelika A Konda; Brandon Brown; Eddy R Segura; Jerome T Galea; Carlos F Caceres; Jeffrey D Klausner
Journal:  BMC Infect Dis       Date:  2013-09-10       Impact factor: 3.090

6.  Phylogenetic analysis of pbp genes in treponemes.

Authors:  Tejpreet Chadha; Adão Alexandre Trindade
Journal:  Infect Ecol Epidemiol       Date:  2013-01-15
  6 in total

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