Literature DB >> 17618608

A novel duplex ELISA method for quantitation of plasma proteins in areas of brain edema.

Matthew C Loftspring1, Joseph F Clark, Kenneth R Wagner.   

Abstract

We have developed a novel type of duplex enzyme-linked immunosorbent assay (ELISA) for the quantitation of the major plasma proteins, IgG and albumin, in edematous brain tissue. We test this duplex ELISA on our porcine intracerebral hemorrhage (ICH) model and show that it is as accurate and sensitive as independent single ELISAs. This method is useful as a marker of edema in brain tissue and the same design can be applied to other proteins and sample types.

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Year:  2007        PMID： 17618608      PMCID： PMC2958047          DOI： 10.1016/j.brainres.2007.06.019

Source DB:  PubMed          Journal:  Brain Res        ISSN： 0006-8993            Impact factor:   3.252


  16 in total

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2.  The Stroke Data Bank: design, methods, and baseline characteristics.

Authors:  M A Foulkes; P A Wolf; T R Price; J P Mohr; D B Hier
Journal:  Stroke       Date:  1988-05       Impact factor: 7.914

Review 3.  Update on the international project on pathologic correlates in MS.

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4.  Extravasation of plasma proteins in brain trauma.

Authors:  H M Liu; W Q Sturner
Journal:  Forensic Sci Int       Date:  1988-09       Impact factor: 2.395

5.  Early metabolic alterations in edematous perihematomal brain regions following experimental intracerebral hemorrhage.

Authors:  K R Wagner; G Xi; Y Hua; M Kleinholz; G M de Courten-Myers; R E Myers
Journal:  J Neurosurg       Date:  1998-06       Impact factor: 5.115

6.  Ultra-early clot aspiration after lysis with tissue plasminogen activator in a porcine model of intracerebral hemorrhage: edema reduction and blood-brain barrier protection.

Authors:  K R Wagner; G Xi; Y Hua; M Zuccarello; G M de Courten-Myers; J P Broderick; T G Brott
Journal:  J Neurosurg       Date:  1999-03       Impact factor: 5.115

7.  Volume of intracerebral hemorrhage. A powerful and easy-to-use predictor of 30-day mortality.

Authors:  J P Broderick; T G Brott; J E Duldner; T Tomsick; G Huster
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Review 8.  Neuropathological changes caused by hydrocephalus.

Authors:  M R Del Bigio
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9.  Lobar intracerebral hemorrhage model in pigs: rapid edema development in perihematomal white matter.

Authors:  K R Wagner; G Xi; Y Hua; M Kleinholz; G M de Courten-Myers; R E Myers; J P Broderick; T G Brott
Journal:  Stroke       Date:  1996-03       Impact factor: 7.914

10.  Multiplex bead array assay for detection of 25 soluble cytokines in blister fluid of patients with complex regional pain syndrome type 1.

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Journal:  Mediators Inflamm       Date:  2006       Impact factor: 4.711
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  2 in total

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Review 2.  Relevance of Porcine Stroke Models to Bridge the Gap from Pre-Clinical Findings to Clinical Implementation.

Authors:  Marc Melià-Sorolla; Carlos Castaño; Núria DeGregorio-Rocasolano; Luis Rodríguez-Esparragoza; Antoni Dávalos; Octavi Martí-Sistac; Teresa Gasull
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  2 in total

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