Literature DB >> 17618292

Red-shifted Renilla reniformis luciferase variants for imaging in living subjects.

Andreas Markus Loening1, Anna M Wu, Sanjiv Sam Gambhir.   

Abstract

The use of R. reniformis luciferase (RLuc) as a reporter gene in small-animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 66 nm (547 nm peak) that also had greater stability and higher light emission than native RLuc.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17618292     DOI： 10.1038/nmeth1070

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  97 in total

Review 1.  Probing heterotrimeric G protein activation: applications to biased ligands.

Authors:  Colette Denis; Aude Saulière; Segolene Galandrin; Jean-Michel Sénard; Céline Galés
Journal:  Curr Pharm Des       Date:  2012       Impact factor: 3.116

2.  Deciphering biased-agonism complexity reveals a new active AT1 receptor entity.

Authors:  Aude Saulière; Morgane Bellot; Hervé Paris; Colette Denis; Frédéric Finana; Jonas T Hansen; Marie-Françoise Altié; Marie-Hélène Seguelas; Atul Pathak; Jakob L Hansen; Jean-Michel Sénard; Céline Galés
Journal:  Nat Chem Biol       Date:  2012-05-27       Impact factor: 15.040

3.  Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy.

Authors:  I Y Chen; O Gheysens; S Ray; Q Wang; P Padmanabhan; R Paulmurugan; A M Loening; M Rodriguez-Porcel; J K Willmann; A Y Sheikh; C H Nielsen; G Hoyt; C H Contag; R C Robbins; S Biswal; J C Wu; S S Gambhir
Journal:  Gene Ther       Date:  2010-03-18       Impact factor: 5.250

Review 4.  Where microbiology meets microengineering: design and applications of reporter bacteria.

Authors:  Jan Roelof van der Meer; Shimshon Belkin
Journal:  Nat Rev Microbiol       Date:  2010-07       Impact factor: 60.633

5.  Optimization of enzyme-substrate pairing for bioluminescence imaging of gene transfer using Renilla and Gaussia luciferases.

Authors:  Takahiro Kimura; Kei Hiraoka; Noriyuki Kasahara; Christopher R Logg
Journal:  J Gene Med       Date:  2010-06       Impact factor: 4.565

6.  Immobilizing reporters for molecular imaging of the extracellular microenvironment in living animals.

Authors:  Zuyong Xia; Yun Xing; Jongho Jeon; Young-Pil Kim; Jessica Gall; Anca Dragulescu-Andrasi; Sanjiv S Gambhir; Jianghong Rao
Journal:  ACS Chem Biol       Date:  2011-08-23       Impact factor: 5.100

7.  Luminopsins integrate opto- and chemogenetics by using physical and biological light sources for opsin activation.

Authors:  Ken Berglund; Kara Clissold; Haofang E Li; Lei Wen; Sung Young Park; Jan Gleixner; Marguerita E Klein; Dongye Lu; Joseph W Barter; Mark A Rossi; George J Augustine; Henry H Yin; Ute Hochgeschwender
Journal:  Proc Natl Acad Sci U S A       Date:  2016-01-05       Impact factor: 11.205

Review 8.  Cardiovascular molecular imaging: focus on clinical translation.

Authors:  Ian Y Chen; Joseph C Wu
Journal:  Circulation       Date:  2011-02-01       Impact factor: 29.690

Review 9.  Near-infrared fluorescent nanoprobes for cancer molecular imaging: status and challenges.

Authors:  Xiaoxiao He; Jinhao Gao; Sanjiv Sam Gambhir; Zhen Cheng
Journal:  Trends Mol Med       Date:  2010-10-01       Impact factor: 11.951

10.  Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.

Authors:  Andreas Markus Loening; Timothy David Fenn; Sanjiv Sam Gambhir
Journal:  J Mol Biol       Date:  2007-10-03       Impact factor: 5.469
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.