A Bembenek1, J Li, C Loddenkemper, W Kemmner, H Stein, K D Wernecke, P M Schlag. 1. Department of Surgery and Surgical Oncology, Robert Rössle Clinic at Helios-Klinikum Berlin, Charité University Medicine Berlin, Campus Buch, Lindenberger Weg 80, D13125 Berlin, Germany. andreas.bembenek@charite.de
Abstract
AIM: Our study examined differences in the presence of mature, DC-Lamp+ DC in the SLN and non-SLN according to the extent of metastatic involvement. PATIENTS AND METHODS: Paraffin blocks of the SLN and non-SLN from patients with primary breast cancer who had undergone SLN biopsy and axillary dissection were separated into three groups: (Group A) no tumor cell involvement in the SLN and non-SLN; (Group B) isolated tumor cells or micrometastases in the SLN, and tumor cell-free non-SLN; and (Group C) macrometastases in the SLN. One section of all the SLN and non-SLN was examined with immunohistochemistry using an anti-DC-Lamp-antibody. The densest area occupied by the DC-Lamp+ cells on each slide was quantified and recorded by an electronic imaging system. In this regard, the SLN and non-SLN were compared within the patients of each group using the Wilcoxon signed rank-test (p<0.05). RESULTS: One hundred and fourteen SLN and 1258 non-SLN from 79 patients were examined. A significantly larger area was occupied by the DC-Lamp(+) cells in the SLN compared to the non-SLN in Groups A (p=0.024) and B (p=0.009), whereas no significant difference was found within Group C (p=0.107). CONCLUSIONS: This study suggests that the DC-dependent immune response is altered during the process of metastasis formation and is primarily activated before and during formation of micrometastasis.
AIM: Our study examined differences in the presence of mature, DC-Lamp+ DC in the SLN and non-SLN according to the extent of metastatic involvement. PATIENTS AND METHODS: Paraffin blocks of the SLN and non-SLN from patients with primary breast cancer who had undergone SLN biopsy and axillary dissection were separated into three groups: (Group A) no tumor cell involvement in the SLN and non-SLN; (Group B) isolated tumor cells or micrometastases in the SLN, and tumor cell-free non-SLN; and (Group C) macrometastases in the SLN. One section of all the SLN and non-SLN was examined with immunohistochemistry using an anti-DC-Lamp-antibody. The densest area occupied by the DC-Lamp+ cells on each slide was quantified and recorded by an electronic imaging system. In this regard, the SLN and non-SLN were compared within the patients of each group using the Wilcoxon signed rank-test (p<0.05). RESULTS: One hundred and fourteen SLN and 1258 non-SLN from 79 patients were examined. A significantly larger area was occupied by the DC-Lamp(+) cells in the SLN compared to the non-SLN in Groups A (p=0.024) and B (p=0.009), whereas no significant difference was found within Group C (p=0.107). CONCLUSIONS: This study suggests that the DC-dependent immune response is altered during the process of metastasis formation and is primarily activated before and during formation of micrometastasis.
Authors: Kim M van Pul; Ronald J C L M Vuylsteke; Rieneke van de Ven; Elisabeth A Te Velde; Emiel J Th Rutgers; Petrousjka M van den Tol; Hein B A C Stockmann; Tanja D de Gruijl Journal: J Immunother Cancer Date: 2019-05-22 Impact factor: 13.751
Authors: Joanna Szpor; Joanna Streb; Anna Glajcar; Piotr Sadowski; Anna Streb-Smoleń; Robert Jach; Diana Hodorowicz-Zaniewska Journal: Int J Mol Sci Date: 2022-07-30 Impact factor: 6.208