OBJECTIVES: The characterization of a Salmonella Infantis strain collection that was set up from isolates of animal and human origin obtained in Hungary in recent years. METHODS: All isolates were phage typed. Antimicrobial resistance was tested by the disc diffusion method, while the presence of the antimicrobial resistance genes and class 1 integrons was investigated by PCR. Genetic relatedness of the isolates was tested by PFGE and plasmid profiling. RESULTS: The majority of the isolates representing different parts of Hungary are characterized by phage types 213 and 217 and the nalidixic acid-streptomycin-sulphonamide-tetracycline resistance type. They harbour a class 1 integron with an aadA1 gene in the 855 bp variable region, a tet(A) gene, a >168 kb plasmid and 66% of them represent one genetic clone as determined by XbaI PFGE fingerprinting. CONCLUSIONS: It seems that broiler chickens constitute a reservoir for one large and a few smaller multidrug-resistant Salmonella Infantis clones in Hungary, which might have spread to humans through chicken meat.
OBJECTIVES: The characterization of a Salmonella Infantis strain collection that was set up from isolates of animal and human origin obtained in Hungary in recent years. METHODS: All isolates were phage typed. Antimicrobial resistance was tested by the disc diffusion method, while the presence of the antimicrobial resistance genes and class 1 integrons was investigated by PCR. Genetic relatedness of the isolates was tested by PFGE and plasmid profiling. RESULTS: The majority of the isolates representing different parts of Hungary are characterized by phage types 213 and 217 and the nalidixic acid-streptomycin-sulphonamide-tetracycline resistance type. They harbour a class 1 integron with an aadA1 gene in the 855 bp variable region, a tet(A) gene, a >168 kb plasmid and 66% of them represent one genetic clone as determined by XbaI PFGE fingerprinting. CONCLUSIONS: It seems that broiler chickens constitute a reservoir for one large and a few smaller multidrug-resistant Salmonella Infantis clones in Hungary, which might have spread to humans through chicken meat.
Authors: Ferenc Olasz; Tibor Nagy; Móni Szabó; János Kiss; Ama Szmolka; Endre Barta; Andries van Tonder; Nicholas Thomson; Paul Barrow; Béla Nagy Journal: Genome Announc Date: 2015-02-12
Authors: Antonio Camarda; Nicola Pugliese; Antonia Pupillo; Marta Oliva; Elena Circella; Anna Maria Dionisi; Antonia Ricci; Marilisa Legretto; Anna Caroli; Carlo Pazzani Journal: Int J Environ Res Public Health Date: 2013-08-06 Impact factor: 3.390