BACKGROUND: Despite numerous studies that have investigated the cellular and molecular mechanisms underlying scar formation, this process still remains poorly understood. The importance of transforming growth factor-beta (TGF-beta) in these processes has been well recognized, and this study sought to define the temporal expression of the key members in this pathway in a well-established, clinically relevant, rabbit ear model of hypertrophic scarring. STUDY DESIGN: Seven-millimeter (hypertrophic) and 5-mm (nonhypertrophic) punch wounds were made on the ears of 12 rabbits. Wounds were harvested at days 0, 7, 15, 28, and 40. RESULTS: There were no appreciable histologic differences between the 5- and 7-mm wounds at days 7 and 15. At day 28, however, the 7-mm scars were considerably more hypertrophic compared with the 5-mm control scars (p<0.001). The mRNA levels of TGF-beta1 and collagen Ialpha2 were notably higher in the hypertrophic 7-mm scars at day 28 than in the nonhypertrophic 5-mm scars (p<0.03). Although not pronounced, levels of TGF-beta2 were higher in the hypertrophic scars. There were no other statistically significant differences between the 7- and 5-mm scars. CONCLUSIONS: Elevated levels of TGF-beta1, and possibly TGF-beta2, are associated with hypertrophic scar formation.
BACKGROUND: Despite numerous studies that have investigated the cellular and molecular mechanisms underlying scar formation, this process still remains poorly understood. The importance of transforming growth factor-beta (TGF-beta) in these processes has been well recognized, and this study sought to define the temporal expression of the key members in this pathway in a well-established, clinically relevant, rabbit ear model of hypertrophic scarring. STUDY DESIGN: Seven-millimeter (hypertrophic) and 5-mm (nonhypertrophic) punch wounds were made on the ears of 12 rabbits. Wounds were harvested at days 0, 7, 15, 28, and 40. RESULTS: There were no appreciable histologic differences between the 5- and 7-mm wounds at days 7 and 15. At day 28, however, the 7-mm scars were considerably more hypertrophic compared with the 5-mm control scars (p<0.001). The mRNA levels of TGF-beta1 and collagen Ialpha2 were notably higher in the hypertrophic 7-mm scars at day 28 than in the nonhypertrophic 5-mm scars (p<0.03). Although not pronounced, levels of TGF-beta2 were higher in the hypertrophic scars. There were no other statistically significant differences between the 7- and 5-mm scars. CONCLUSIONS: Elevated levels of TGF-beta1, and possibly TGF-beta2, are associated with hypertrophic scar formation.
Authors: Letícia E Charelli; Nathalia Müller; Karina R Silva; Luís Maurício T R Lima; Celso Sant'Anna; Leandra S Baptista Journal: Cytotechnology Date: 2018-11-01 Impact factor: 2.058
Authors: Adam Ponedal; Shengshuang Zhu; Anthony J Sprangers; Xiao-Qi Wang; David C Yeo; Daniel C S Lio; Mengjia Zheng; Matthew Capek; Suguna P Narayan; Brian Meckes; Amy S Paller; Chenjie Xu; Chad A Mirkin Journal: ACS Appl Bio Mater Date: 2020-11-13
Authors: Nick L Occleston; Anthony D Metcalfe; Adam Boanas; Nicholas J Burgoyne; Kerry Nield; Sharon O'Kane; Mark W J Ferguson Journal: Dermatol Res Pract Date: 2010-08-03
Authors: Luciana B Lopes; Elizabeth J Furnish; Padmini Komalavilas; Charles R Flynn; Patricia Ashby; Adam Hansen; Daphne P Ly; George P Yang; Michael T Longaker; Alyssa Panitch; Colleen M Brophy Journal: J Invest Dermatol Date: 2008-09-11 Impact factor: 8.551