Literature DB >> 1761700

Indirect immunofluorescence assay for detection of Helicobacter pylori in human gastric mucosal biopsies.

E Rivera1, Y López-Vidal, V Luqueño, G M Ruiz-Palacios.   

Abstract

To determine sensitivity and specificity of immunofluorescence assay (IFA) for detection of Helicobacter pylori, we studied 151 patients. Biopsies of gastric mucosae were obtained for culture, histological testing, and IFA. H. pylori serum antibodies were tested by enzyme-linked immunosorbent assay. IFA was done on Formalin-preserved, paraffin-embedded biopsies by using rabbit anti-H. pylori and goat anti-rabbit gamma globulin-fluorescein isothiocyanate conjugate. The sensitivity and specificity of IFA compared with culture and Warthin-Starry stain were 93 and 95%, respectively. IFA is an accurate method for the diagnosis of H. pylori infection.

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Mesh：

Year:  1991        PMID： 1761700      PMCID： PMC270198          DOI： 10.1128/jcm.29.8.1748-1751.1991

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Prospective double-blind trial of duodenal ulcer relapse after eradication of Campylobacter pylori.

Authors:  B J Marshall; C S Goodwin; J R Warren; R Murray; E D Blincow; S J Blackbourn; M Phillips; T E Waters; C R Sanderson
Journal:  Lancet       Date:  1988 Dec 24-31       Impact factor: 79.321

2.  Usefulness of culture, histology, and urease testing in the detection of Campylobacter pylori.

Authors:  G A Schnell; T T Schubert
Journal:  Am J Gastroenterol       Date:  1989-02       Impact factor: 10.864

3.  Monoclonal antibodies for rapid identification of Campylobacter pyloridis.

Authors:  L Engstrand; C Pählson; S Gustavsson; A Schwan
Journal:  Lancet       Date:  1986-12-13       Impact factor: 79.321

4.  Pyloric CLO in the human stomach.

Authors:  J R Lambert; K L Dunn; E R Eaves; M G Korman; J Hansky; K J Pinkard
Journal:  Med J Aust       Date:  1985-08-19       Impact factor: 7.738

5.  Assessment of Campylobacter-like organisms in the postoperative stomach, iatrogenic gastritis, and chronic gastroduodenal diseases: preliminary observations.

Authors:  S Gustavsson; S F Phillips; J R Malagelada; J E Rosenblatt
Journal:  Mayo Clin Proc       Date:  1987-04       Impact factor: 7.616

6.  Enzyme-linked immunosorbent assay for Campylobacter pyloridis: correlation with presence of C. pyloridis in the gastric mucosa.

Authors:  C S Goodwin; E Blincow; G Peterson; C Sanderson; W Cheng; B Marshall; J R Warren; R McCulloch
Journal:  J Infect Dis       Date:  1987-03       Impact factor: 5.226

7.  Indirect immunofluorescence test and enzyme-linked immunosorbent assay for detection of Campylobacter pylori.

Authors:  E Schaber; F Umlauft; G Stöffler; F Aigner; B Paulweber; F Sandhofer
Journal:  J Clin Microbiol       Date:  1989-02       Impact factor: 5.948
  7 in total
  1 in total

1.  Evaluation of a monoclonal antibody for detection of Helicobacter pylori in a direct immunofluorescence test.

Authors:  U Rodewig; W Bemb; D Bitter-Suermann; M Elsheikh; S Fritsch; E Glenn-Calvo; B Soudah; M Varrentrapp; S Wagner; W Bär
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-08       Impact factor: 3.267
  1 in total

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