AIM: The normal endovascular invasion of trophoblast cells and spiral artery remodeling are impaired in pre-eclampsia. Neither the circulating factor secreted by the placenta nor the cause of the widespread endothelial dysfunction in pre-eclampsia has yet been identified. In an attempt to identify novel factors, we performed a gene expression profiling study of placental tissue from women with and without pre-eclampsia. MATERIAL AND METHODS: The study group comprised two pre-eclamptic patients with intrauterine growth restriction while the control group comprised three healthy women with uncomplicated pregnancies. Gene expression was studied using Affymetrix Human Genome U133 Plus 2 micro arrays. We focused on genes associated with angiogenesis. Some of the micro array analysis results were verified using real-time reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Gene expression profiling revealed that the expression level of nine genes--ECGF1, JAG1, Palladin, COL18A1, TNFSF12, VEGF, ANPEP, PDGFRA and SERPIN12 - was downregulated whereas the level of four genes--EPAS1, FLT1, SIGLE10 and ANG4--was upregulated in the study group compared with the control group. The real-time RT-PCR results from JAG1, COL18A1 and FLT1 genes were in accordance with the gene expression results. CONCLUSION: Our results show new targets for research to understand the mechanisms leading to pre-eclampsia.
AIM: The normal endovascular invasion of trophoblast cells and spiral artery remodeling are impaired in pre-eclampsia. Neither the circulating factor secreted by the placenta nor the cause of the widespread endothelial dysfunction in pre-eclampsia has yet been identified. In an attempt to identify novel factors, we performed a gene expression profiling study of placental tissue from women with and without pre-eclampsia. MATERIAL AND METHODS: The study group comprised two pre-eclamptic patients with intrauterine growth restriction while the control group comprised three healthy women with uncomplicated pregnancies. Gene expression was studied using Affymetrix Human Genome U133 Plus 2 micro arrays. We focused on genes associated with angiogenesis. Some of the micro array analysis results were verified using real-time reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Gene expression profiling revealed that the expression level of nine genes--ECGF1, JAG1, Palladin, COL18A1, TNFSF12, VEGF, ANPEP, PDGFRA and SERPIN12 - was downregulated whereas the level of four genes--EPAS1, FLT1, SIGLE10 and ANG4--was upregulated in the study group compared with the control group. The real-time RT-PCR results from JAG1, COL18A1 and FLT1 genes were in accordance with the gene expression results. CONCLUSION: Our results show new targets for research to understand the mechanisms leading to pre-eclampsia.
Authors: A Rajakumar; T Chu; D E Handley; K D Bunce; B Burke; C A Hubel; A Jeyabalan; D G Peters Journal: Placenta Date: 2010-11-13 Impact factor: 3.481
Authors: S Brouillet; P Murthi; P Hoffmann; A Salomon; F Sergent; P De Mazancourt; M Dakouane-Giudicelli; M N Dieudonné; P Rozenberg; D Vaiman; S Barbaux; M Benharouga; J-J Feige; N Alfaidy Journal: Cell Mol Life Sci Date: 2012-09-02 Impact factor: 9.261
Authors: Matthew A Maccani; James F Padbury; Barry M Lester; Valerie S Knopik; Carmen J Marsit Journal: Pediatr Res Date: 2013-06-19 Impact factor: 3.756