Literature DB >> 17616601

Artificial control of nitrate respiration through the lac promoter permits the assessment of oxygen-mediated posttranslational regulation of the nar operon in Pseudomonas aeruginosa.

Chris E Noriega1, Vandana Sharma, John J Rowe.   

Abstract

In this study, oxygen and nitrate regulation of transcription and subsequent protein expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to assess both transcriptional and posttranslational regulation by oxygen during physiological whole-cell nitrate reduction.

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Year:  2007        PMID： 17616601      PMCID： PMC1951927          DOI： 10.1128/JB.00491-07

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  40 in total

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Authors:  M W Pfaffl
Journal:  Nucleic Acids Res       Date:  2001-05-01       Impact factor: 16.971

2.  Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria.

Authors:  Christopher J Marx; Mary E Lidstrom
Journal:  Biotechniques       Date:  2002-11       Impact factor: 1.993

3.  Mapping and characerization of mutants of Pseudomonas aeruginosa affected in nitrate respiration in aerobic or anaerobic growth.

Authors:  J van Hartingsveldt; A H Stouthamer
Journal:  J Gen Microbiol       Date:  1973-01

4.  Purification and properties of nitrate reductase from Escherichia coli K12.

Authors:  C H MacGregor; C A Schnaitman; D E Normansell
Journal:  J Biol Chem       Date:  1974-08-25       Impact factor: 5.157

Review 5.  Anaerobic metabolism and quorum sensing by Pseudomonas aeruginosa biofilms in chronically infected cystic fibrosis airways: rethinking antibiotic treatment strategies and drug targets.

Authors:  Daniel J Hassett; John Cuppoletti; Bruce Trapnell; Sergei V Lymar; John J Rowe; Sang Sun Yoon; George M Hilliard; Kislay Parvatiyar; Moneesha C Kamani; Daniel J Wozniak; Sung Hei Hwang; Timothy R McDermott; Urs A Ochsner
Journal:  Adv Drug Deliv Rev       Date:  2002-12-05       Impact factor: 15.470

6.  Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR.

Authors:  Hakan Savli; Aynur Karadenizli; Fetiye Kolayli; Sibel Gundes; Ugur Ozbek; Haluk Vahaboglu
Journal:  J Med Microbiol       Date:  2003-05       Impact factor: 2.472

7.  Long-term anaerobic survival of the opportunistic pathogen Pseudomonas aeruginosa via pyruvate fermentation.

Authors:  Martin Eschbach; Kerstin Schreiber; Katharina Trunk; Jan Buer; Dieter Jahn; Max Schobert
Journal:  J Bacteriol       Date:  2004-07       Impact factor: 3.490

8.  Biochemical Society Special Lecture. Nitrate- and nitrite-responsive sensors NarX and NarQ of proteobacteria.

Authors:  V Stewart
Journal:  Biochem Soc Trans       Date:  2003-02       Impact factor: 5.407

9.  Expression of the nir and nor genes for denitrification of Pseudomonas aeruginosa requires a novel CRP/FNR-related transcriptional regulator, DNR, in addition to ANR.

Authors:  H Arai; Y Igarashi; T Kodama
Journal:  FEBS Lett       Date:  1995-08-28       Impact factor: 4.124

10.  Nitrate sensing and metabolism modulate motility, biofilm formation, and virulence in Pseudomonas aeruginosa.

Authors:  Nadine E Van Alst; Kristin F Picardo; Barbara H Iglewski; Constantine G Haidaris
Journal:  Infect Immun       Date:  2007-05-25       Impact factor: 3.441
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  1 in total

1.  Mini-Tn7 vectors for stable expression of diguanylate cyclase PleD* in Gram-negative bacteria.

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Journal:  BMC Microbiol       Date:  2015-09-29       Impact factor: 3.605
  1 in total

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