Novel chemiluminescence system with calcein as chemiluminescent reagent.

Chemiluminescence (CL) was observed when calcein reacted with potassium ferricyanide. Several metal ions and organic compounds could enhance this CL signal. The possible mechanisms for the CL reactions and the enhancement effect were investigated via studies of the CL kinetic characteristics, the CL spectra of some related reactions, the UV absorption spectra and the fluorescence spectra of some substances. The merits of figures for some substances with CL activity were obtained. A new CL system with calcein as the CL reagent was preliminarily established. Copyright 2007 John Wiley & Sons, Ltd.