BACKGROUND: This study was designed to investigate the relationship between prenatal pesticide exposures and the generation of leukemia-associated t(8;21)(q22;q22), one of the most common cytogenetic abnormalities in childhood acute myeloid leukemia (AML). PROCEDURE: Gas chromatography/mass spectrometry (GC/MS) was used to quantitatively detect different pesticides (propoxur and cypermethrin) in meconium from 49 newborn babies from the Philippines. The generation of t(8;21) was determined in the corresponding umbilical cord blood samples by detection of the AML1-ETO fusion transcripts derived from t(8;21) using nested RT-PCR. Levels for the AML1-ETO fusion transcripts were quantitated by real-time RT-PCR in the t(8;21) positive cord blood samples. AML1-ETO fusion transcript forms were characterized by RT-PCR amplification and DNA sequencing. RESULTS: In the present study using umbilical cord blood samples obtained from infants whose prenatal exposure to the pesticide, propoxur, was determined by meconium analysis, we showed that (i) incidence of t(8;21) in the exposed group was two-fold higher than that in the unexposed group; and (ii) the levels for AML1-ETO fusion transcripts resulting from t(8;21) positively correlated with propoxur concentrations in meconium. Similar heterogeneity in the fusion transcripts was detected in the t(8;21) positive cord blood samples as in our previous study with t(8;21) AML patients. CONCLUSION: These results further confirm the prenatal origin of t(8;21) and establish a significant correlation between prenatal pesticide exposures and the generation of t(8;21). They suggest that prenatal pesticide exposures may be causal factors for the generation of leukemia-associated chromosomal translocations. (c) 2007 Wiley-Liss, Inc.
BACKGROUND: This study was designed to investigate the relationship between prenatal pesticide exposures and the generation of leukemia-associated t(8;21)(q22;q22), one of the most common cytogenetic abnormalities in childhood acute myeloid leukemia (AML). PROCEDURE: Gas chromatography/mass spectrometry (GC/MS) was used to quantitatively detect different pesticides (propoxur and cypermethrin) in meconium from 49 newborn babies from the Philippines. The generation of t(8;21) was determined in the corresponding umbilical cord blood samples by detection of the AML1-ETO fusion transcripts derived from t(8;21) using nested RT-PCR. Levels for the AML1-ETO fusion transcripts were quantitated by real-time RT-PCR in the t(8;21) positive cord blood samples. AML1-ETO fusion transcript forms were characterized by RT-PCR amplification and DNA sequencing. RESULTS: In the present study using umbilical cord blood samples obtained from infants whose prenatal exposure to the pesticide, propoxur, was determined by meconium analysis, we showed that (i) incidence of t(8;21) in the exposed group was two-fold higher than that in the unexposed group; and (ii) the levels for AML1-ETO fusion transcripts resulting from t(8;21) positively correlated with propoxur concentrations in meconium. Similar heterogeneity in the fusion transcripts was detected in the t(8;21) positive cord blood samples as in our previous study with t(8;21) AMLpatients. CONCLUSION: These results further confirm the prenatal origin of t(8;21) and establish a significant correlation between prenatal pesticide exposures and the generation of t(8;21). They suggest that prenatal pesticide exposures may be causal factors for the generation of leukemia-associated chromosomal translocations. (c) 2007 Wiley-Liss, Inc.
Authors: Offie P Soldin; Hala Nsouli-Maktabi; Hala Nsouly-Maktabi; Jeanine M Genkinger; Christopher A Loffredo; Juan Antonio Ortega-Garcia; Drew Colantino; Dana B Barr; Naomi L Luban; Aziza T Shad; David Nelson Journal: Ther Drug Monit Date: 2009-08 Impact factor: 3.681
Authors: K M LaFiura; H Edwards; J W Taub; L H Matherly; J A Fontana; A N Mohamed; Y Ravindranath; Y Ge Journal: Oncogene Date: 2008-05-12 Impact factor: 9.867
Authors: Helen D Bailey; Lin Fritschi; Claire Infante-Rivard; Deborah C Glass; Lucia Miligi; John D Dockerty; Tracy Lightfoot; Jacqueline Clavel; Eve Roman; Logan G Spector; Peter Kaatsch; Catherine Metayer; Corrado Magnani; Elizabeth Milne; Sophia Polychronopoulou; Jill Simpson; Jérémie Rudant; Vasiliki Sidi; Roberto Rondelli; Laurent Orsi; Alice Y Kang; Eleni Petridou; Joachim Schüz Journal: Int J Cancer Date: 2014-04-04 Impact factor: 7.396