PURPOSE: In a previous immunohistochemical study of dendritic cells (DC) in sentinel lymph nodes (SLN) draining regressing melanomas, we found that the accumulation of mature DC-LAMP(+) DCs in SLNs was associated with local expansion of antigen-specific memory effector CTLs and the absence of metastasis in downstream lymph nodes. The aim of this study was to investigate the prognostic importance of the maximal density of mature DCs in SLNs. EXPERIMENTAL DESIGN: A total of 458 consecutive patients with micrometastatic melanoma within SLNs were eligible for analysis. The maximal density of mature DC-LAMP(+) DCs was evaluated by three independent observers and categorized into three classes (<100, 100 to <200, and >or=200/mm(2)). RESULTS: There was excellent interobserver reproducibility for maximum density of mature DC-LAMP(+) DC scores (kappa score = 0.82). There were differences in the maximal density scores and staining intensity according to the treating melanoma center (P < 0.001). The higher the mature DC density in the SLN is, the longer is the duration of survival [P = 0.047; hazard ratio, 0.70; 95% confidence interval, 0.50-1.00]. Adjusted by thickness and ulceration, the prognostic importance of DC density was lower (P = 0.36). CONCLUSION: This study is the first to report the prognostic value of DC-LAMP(+) DC counts in SLNs containing metastatic melanoma. Patients with a high density of mature DCs (>or=200/mm(2)) have the lowest risk of death. It also provides evidence that a lack of maturation in the SLNs is important in biological facilitation of melanoma progression.
PURPOSE: In a previous immunohistochemical study of dendritic cells (DC) in sentinel lymph nodes (SLN) draining regressing melanomas, we found that the accumulation of mature DC-LAMP(+) DCs in SLNs was associated with local expansion of antigen-specific memory effector CTLs and the absence of metastasis in downstream lymph nodes. The aim of this study was to investigate the prognostic importance of the maximal density of mature DCs in SLNs. EXPERIMENTAL DESIGN: A total of 458 consecutive patients with micrometastatic melanoma within SLNs were eligible for analysis. The maximal density of mature DC-LAMP(+) DCs was evaluated by three independent observers and categorized into three classes (<100, 100 to <200, and >or=200/mm(2)). RESULTS: There was excellent interobserver reproducibility for maximum density of mature DC-LAMP(+) DC scores (kappa score = 0.82). There were differences in the maximal density scores and staining intensity according to the treating melanoma center (P < 0.001). The higher the mature DC density in the SLN is, the longer is the duration of survival [P = 0.047; hazard ratio, 0.70; 95% confidence interval, 0.50-1.00]. Adjusted by thickness and ulceration, the prognostic importance of DC density was lower (P = 0.36). CONCLUSION: This study is the first to report the prognostic value of DC-LAMP(+) DC counts in SLNs containing metastatic melanoma. Patients with a high density of mature DCs (>or=200/mm(2)) have the lowest risk of death. It also provides evidence that a lack of maturation in the SLNs is important in biological facilitation of melanoma progression.
Authors: Paolo A Ascierto; Antonio M Grimaldi; Nicolas Acquavella; Lorenzo Borgognoni; Luana Calabrò; Natale Cascinelli; Alessandra Cesano; Michele Del Vecchio; Alexander M Eggermont; Mark Faries; Soldano Ferrone; Bernard A Fox; Thomas F Gajewski; Jérôme Galon; Sacha Gnjatic; Helen Gogas; Mohammed Kashani-Sabet; Howard L Kaufman; James Larkin; Roger S Lo; Alberto Mantovani; Kim Margolin; Cornelis Melief; Grant McArthur; Giuseppe Palmieri; Igor Puzanov; Antoni Ribas; Barbara Seliger; Jeff Sosman; Peter Suenaert; Ahmad A Tarhini; Giorgio Trinchieri; Fernando Vidal-Vanaclocha; Ena Wang; Gennaro Ciliberto; Nicola Mozzillo; Francesco M Marincola; Magdalena Thurin Journal: J Transl Med Date: 2013-06-03 Impact factor: 5.531
Authors: Nicolas Jacquelot; María Paula Roberti; David P Enot; Sylvie Rusakiewicz; Michaela Semeraro; Sarah Jégou; Camila Flores; Lieping Chen; Byoung S Kwon; Christophe Borg; Benjamin Weide; François Aubin; Stéphane Dalle; Holbrook Kohrt; Maha Ayyoub; Guido Kroemer; Aurélien Marabelle; Andréa Cavalcanti; Alexander Eggermont; Laurence Zitvogel Journal: J Invest Dermatol Date: 2016-01-29 Impact factor: 8.551
Authors: H Gogas; A M M Eggermont; A Hauschild; P Hersey; P Mohr; D Schadendorf; A Spatz; R Dummer Journal: Ann Oncol Date: 2009-08 Impact factor: 32.976
Authors: Rosalyn Jewell; Faye Elliott; Jonathan Laye; Jérémie Nsengimana; John Davies; Christy Walker; Caroline Conway; Angana Mitra; Mark Harland; Martin G Cook; Andy Boon; Sarah Storr; Sabreena Safuan; Stewart G Martin; Karin Jirström; Håkan Olsson; Christian Ingvar; Martin Lauss; Tim Bishop; Göran Jönsson; Julia Newton-Bishop Journal: Pigment Cell Melanoma Res Date: 2014-10-01 Impact factor: 4.693