Literature DB >> 17606304

Immunogenicity of plasmids encoding P12A and 3C of FMDV and swine IL-18.

Ma Mingxiao1, Jin Ningyi, Liu Hui Juan, Zheng Min, Shen Guoshun, Zhu Guangze, Lu Huijun, Huo Xiaowei, Jin Minglan, Li Xu, Ma Haili, Ji Yue, Yin Gefen, Jin Kuoshi.   

Abstract

In this paper, two recombinant plasmids (pVIR-P12AIL18-3C and pVIR-P12A-3C) containing foot and mouth disease virus (FMDV) capsid polypeptide, 3C coding regions of O/NY00 and using/or not swine IL18 as a genetic adjuvant were constructed, and evaluated for their ability to induce humoral and cellular responses in mice and swine. In addition, the ability to protect swine against homologous virus challenge was examined. Mice and swine were given booster vaccination twice and once, respectively, and swine were challenged 10 days after the booster vaccination. Control groups were inoculated with pVAX1 or phosphate-buffered saline (PBS). All animals vaccinated with pVIR-P12AIL18-3C and pVIR-P12A-3C developed specific anti-FMDV ELISA antibody and neutralizing antibody and T lymphocyte proliferation and CTL cytotoxic activity was observed. In addition, we found that pVIR-P12AIL18-3C possessed stronger immunogenicity than pVIR-P12A-3C. The pVIR-P12AIL18-3C and pVIR-P12A-3C provided full protection in 3/4 and 2/4 swine from challenge with FMDV O/NY00, respectively. The results demonstrate the potential viability of a DNA vaccine in the control and prevention of FMDV infections.

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Year:  2007        PMID： 17606304     DOI： 10.1016/j.antiviral.2007.05.003

Source DB:  PubMed          Journal:  Antiviral Res        ISSN： 0166-3542            Impact factor:   5.970


  5 in total

1.  Immune responses of piglets immunized by a recombinant plasmid containing porcine circovirus type 2 and porcine interleukin-18 genes.

Authors:  Guang-Lei Chen; Peng-Fei Fu; Lin-Qing Wang; Xin-Sheng Li; Hong-Ying Chen
Journal:  Viral Immunol       Date:  2014-12       Impact factor: 2.257

2.  Co-expression of the Bcl-xL antiapoptotic protein enhances the induction of Th1-like immune responses in mice immunized with DNA vaccines encoding FMDV B and T cell epitopes.

Authors:  Sultan Gülçe İz; Mert Döşkaya; Belen Borrego; Fernando Rodriguez; Yüksel Gürüz; Ismet Deliloğlu Gürhan
Journal:  Vet Res Commun       Date:  2013-03-13       Impact factor: 2.459

3.  Comparison of immune responses against FMD by a DNA vaccine encoding the FMDV/O/IRN/2007 VP1 gene and the conventional inactivated vaccine in an animal model.

Authors:  Farahnaz Motamedi Sedeh; Hoorieh Soleimanjahi; AmirReza Jalilian; Homayoon Mahravani
Journal:  Virol Sin       Date:  2012-09-21       Impact factor: 4.327

4.  Intranasal delivery of cationic PLGA nano/microparticles-loaded FMDV DNA vaccine encoding IL-6 elicited protective immunity against FMDV challenge.

Authors:  Gang Wang; Li Pan; Yongguang Zhang; Yonglu Wang; Zhongwang Zhang; Jianliang Lü; Peng Zhou; Yuzhen Fang; Shoutian Jiang
Journal:  PLoS One       Date:  2011-11-15       Impact factor: 3.240

5.  Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.

Authors:  Ana Clara Mignaqui; Vanesa Ruiz; Sylvie Perret; Gilles St-Laurent; Parminder Singh Chahal; Julia Transfiguracion; Ayelén Sammarruco; Victoria Gnazzo; Yves Durocher; Andrés Wigdorovitz
Journal:  PLoS One       Date:  2013-08-20       Impact factor: 3.240
  5 in total

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